牛免疫缺陷病毒(BIV)在中美两国流行情况的比较(英文)  

Comparative Studies on BIV Infection in China and the USA

作　　者：王金忠[1,2] 刘淑红[1,2] 陈启民[1,2] 耿运琪[1,2] 

机构地区：[1]南开大学生命科学院 [2]美国内布拉斯加大学生命科学学院

出　　处：《河北大学学报（自然科学版）》1999年第2期170-175,共6页Journal of Hebei University(Natural Science Edition)

摘　　要：牛免疫缺陷病毒是反转录病毒科慢病毒属的重要成员，已在一些国家相继发现ＢＩＶ的感染和蔓延。本文以重组ＢＩＶ衣壳蛋白和包膜蛋白为抗原，通过免疫印迹技术发现，不仅我国进口奶牛及其后代存在一定比例的ＢＩＶ感染（血清阳性率为２．３２％），而且ＢＩＶ已开始在我国北方牛群中传播（阳性率约３．８％）。上述结果已被聚合酶链式反应及其产物的狭缝杂交所证实，并与美国中部及南部地区ＢＩＶ流行情况进行了比较。Bovine immunodeficiency virus(BIV) is a bovine lentivirus that infects animals worldwide. The BIV gag and env genes, as well as several sub_regions spanning the entire open reading frames coding these two proteins, were cloned and expressed as fusions to the TrpE protein in E. coli. These recombinant proteins could readily be expressed, but the expression levels of these piptides varied. To screen for naturally_infected cattle, the bacterial recombinant BIV gag and env proteins have been used as a source of antigens to detect anti_BIV antibodies by western blot analyses. Approximate 3.8% of sera from the northern counties of Hebei Province were BIV positive, while the seroprevalence of BIV was about 2.32% among imported cows and their progeny in North China. The 3%4% seropositive rate was similar to that reported in the southern and southwestern regions of the US, but it was lower than average frequencies in Louisiana, Mississippi or central USA states. The screening results were also confirm

关 键 词：免疫缺陷病毒 融合蛋白 感染 BIV 牛病毒 

分 类 号：S852.653[农业科学—基础兽医学]