HLA-Ⅰ基因型对HIV-1感染患儿特异性细胞毒性T细胞应答的影响  

Impacts of HLA-Ⅰ on specific cytotoxic T lymphocyte responses in HIV-1-infected children

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作  者:占晗琳[1] 唐漾波[1] 赵稳[1] 唐小平[1] 

机构地区:[1]广州医学院附属市八人民医院传染病研究所,510060

出  处:《国际流行病学传染病学杂志》2010年第4期223-226,共4页International Journal of Epidemiology and Infectious Disease

基  金:广州市医药卫生科技重点项目(2006-ZDi-05);广州市科技计划项目(2007J1-C0191)

摘  要:目的 对HIV-1感染患儿的人类白细胞抗原(HLA)-Ⅰ基因型进行分析,探讨其对HIV-1特异性CTL应答的影响.方法 采用酶联免疫斑点技术(ELISPOT),以HIV-1 P24区域的氨基酸序列人工合成的12个重叠肽段组成的肽段库作为特异性抗原表位,对5例接受HAART后的HIV-1感染患儿外周血单核细胞(PBMC)的IFN-γ分泌细胞频数进行测定研究.同时,还采用了PCR-序列特异引物技术(PCR-SSP)及PCR-直接碱基序列分析基因分型技术(PCR-SBT)对这5例患儿进行了HLA-Ⅰ等位基因分型及HLA-B*高分辨等位基因分型.结果 5例HIV-1感染息儿中,4例HLA-B*基因型为B*40,其中3例为HLA-B*4001,1例为HLA-B*4002.其HIV-1抗原特异性CTL应答水平差异明显.5例患儿均未出现免疫重建炎症综合征.结论 基因型为HLA-B*40的儿童可能更易感染HIV-1,HLA-B*40可能影响HIV-1感染患儿的抗原特异性CTL应答.Objective To analyze human leucocyte antigen-Ⅰ (HLA-Ⅰ)genotype of HIV-1-infected-children and explore the influence of HLA-Ⅰ on HIV-1 specific cytotoxic T lymphocyte (CTL)responses. Methods Frequency of IFN-γ secreting cells in PBMC from 5 HAART-treated HIV-1-infected children were assessed by stimulation with a peptide pool containing 12 overlapping peptdes in HIV-1 P24, by using assay of enzyme-linked immunospot (ELISPOT).Moreover, HLA-Ⅰ alleles of them were genotyped by assays of polymerase chain reaction-sequence specific primer (PCRSSP) and high-resolution subtyping using PCR-sequence based genotyping (PCR-SBT). Results Among 5 HIV-1infected-children, there were 4 cases with HLA-B* genotype, including 3 cases with HLA-B* 4001 and 1 case with HLA-B* 4002. Frequency of HIV-1 antigen specific CTL responses existed obvious differences. None of 5 HIV-1 infected-children developed immune reconstitution inflammtory syndrome. Conclusions Children with HLA-B* 40genotype may be susceptible to HIV-1. HLA-B* 40 may affect HIV-1 specific CTL responses in HIV-1-infected children.

关 键 词:HIV-1 人类白细胞抗原Ⅰ 细胞毒性T细胞 HAART 酶联免疫斑点技术 

分 类 号:R725.1[医药卫生—儿科]

 

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