一株分离自高海拔土壤的梭菌的鉴定及代谢产物  

Identification and metabolism characterization of a Clostridium lituseburense strain isolated from high-altitude soil

作　　者：刘全全[1] 刘海昌[1] 邓宇[1] 胡国全[1] 张辉[1] 

机构地区：[1]农业部沼气科学研究所,农业部能源微生物与利用重点开放实验室

出　　处：《微生物学报》2010年第9期1135-1140,共6页Acta Microbiologica Sinica

基　　金：国家科技支撑计划(2008BADC4B04);国家“863计划”(2007AA100705)~~

摘　　要：【目的】了解土壤环境中细菌的生理生化特性及代谢产物。【方法】采用厌氧操作技术从西藏纳木错分离到一株厌氧梭状芽孢杆菌P4-1。采用生理生化鉴定结合16SrDNA序列的系统发育学分析确定该菌株的系统发育地位,用气相色谱分析其代谢产物【结果】菌株P4-1为严格厌氧革兰氏阳性菌,菌体大小为0.3μm×1μm-3μm,单生或成对生长,产生芽孢。生长温度范围是13-40℃（最适温度37℃）;NaCl浓度0%-5%（最适为0.5%-1%）;pH范围5.0-10.0（最适生长pH为7.5-8.0）。能够利用葡萄糖、密二糖、甘露糖醇等多种碳水化合物,发酵葡萄糖的产物是乙酸,丙酸,丁酸,CO2,H2。菌株P4-1的（G＋C）mol%含为30.9%,与Clostridium lituseburense DSM797（M59107）的相似性达98.7%。菌株P4-1能够降解对甲苯磺酸盐,并且在有酵母粉的情况下能明显提高其降解能力【结论】菌株P4-1是从土壤中分离出的一株耐低温,耐盐的厌氧菌,其发酵葡萄糖产生的代谢产物有利于改善土壤中的微环境,而且能够降解对甲苯磺酸盐,在废水处理中有重要意义。[Objective]We studied the physiological,biochemical properties and metabolism of Clostridium lituseburense P4-1 from soil in Namucuo.[Methods]We adopted Hungate anaerobic technique to get Strain P4-1 from soil in Namucuo.Through physiological,biochemical and phylogenetic analysis,we identified the strain P4-1.[Results]Cells were Gram-positive and spore-forming.It grew between 13 and 40℃（optimum at 37℃）,between pH value 5.0 and 10.0（optimum at 7.5-8.0）,and with the presence of NaCl between 0%-5%.Strain P4-1 could metabolize many carbon sources including glucose,melibiose and mannitol.Metabolites of glucose were acetate,butyrate,propionate,CO2,and little H2.Based on 16S rDNA studies,strain P4-1 was most close to Clostridium lituseburense DSM 797（M59107） with 98.7% similarity.Strain P4-1 could degrade p-toluene sulfonate.[Conclusion]Strain P4-1 tolerated low temperature,salt and could degrade p-toluene sulfonate.Its metabolites produced by fermentation of glucose could improve the soil micro-environment.It was significant for strain P4-1 to be utilized in the wastewater treatment.

关 键 词：象牙海岸梭菌 鉴定 对甲苯磺酸盐 

分 类 号：Q93-3[生物学—微生物学]