木犀草素对金黄色葡萄球菌的抑菌活性及其机制  

Antibacterial activity and mechanism of Luteolin on Staphylococcus aureus

作　　者：王倩[1] 谢明杰[1] 

机构地区：[1]辽宁师范大学生命科学学院

出　　处：《微生物学报》2010年第9期1180-1184,共5页Acta Microbiologica Sinica

基　　金：辽宁省自然科学基金(20072153);大连市科技计划项目(2009E12SF165)~~

摘　　要：【目的】研究木犀草素对金黄色葡萄球菌的抑制活性及其机制。【方法】利用2,3,5-氯化三苯基四氮唑（TTC）染色,细胞膜渗透性测定,SDS-PAGE蛋白谱变化,4′,6-二脒基-2-苯基吲哚（DAPI）荧光染色法等对木犀草素的抑菌活性及其机制进行研究。【结果】木犀草素能影响金黄色葡萄球菌细胞膜的通透性,木犀草素作用16h,菌体可溶性蛋白总量减少64.54%,DNA含量减少48.44%,RNA含量减少39.35%,木犀草素的浓度为1.6mg/mL时,拓扑异构酶I和II的活性可完全被抑制。【结论】木犀草素有明显的抑菌活性,其抑菌机制主要是通过抑制DNA拓扑异构酶的活性,进而影响菌体核酸及蛋白质的合成来实现的。[Objective]To investigate the antibacterial activity and mechanism of luteolin on Staphylococcus aureus.[Methods]The antibacterial activity and mechanism experiments were carried out by using 2,3,5-triphenyl tetrazolium chloride（TTC）,determining membrane penetrability,the change of SDS-PAGE protein spectra and 4＇6-diamidino-2phenylindole（DAPI） staining assay.[Results] Luteolin could affect the membrane permeability of Staphylococcus aureus,but not destroy the membrane integrity directly.After treated with luteolin for 16 hours,the total content of proteins decreased to 64.54%,the quantity of both DNA and RNA reduced to 48.44% and 39.35% respectively.The activity of DNA topoisomerase I and II was inhibited completely by 1.6 mg /mL luteolin.[Conclusion]Luteolin showed obvious antibacterial activity against Staphylococcus aureus.The antibacterial mechanism of luteolin is that it could inhibit the activity of DNA topoisomerase I and II,which resulted in some decrease in the nucleic acid and protein synthesis.

关 键 词：木犀草素 抑菌机制 DNA拓扑异构酶 

分 类 号：R285[医药卫生—中药学]