乳酸杆菌S-层蛋白对鼠伤寒沙门氏菌黏附及入侵Caco-2细胞的拮抗作用  

Effects of S-layer proteins from lactobacillus against Salmonella typhimurium adhesion and invasion on Caco-2 cells

作　　者：李鹏成[1] 叶小兰[1] 王志胜[1] 庾庆华[1] 杨倩[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《微生物学报》2010年第9期1226-1231,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目(30871858);江苏省支撑计划(BE200830155);教育部博士点基金(B200606)~~

摘　　要：【目的】对嗜酸乳杆菌的S-层蛋白（S-layer protein）进行提纯,研究嗜酸乳酸杆菌和S-层蛋白对鼠伤寒沙门氏菌黏附和入侵的拮抗作用。【方法】应用阴离子交换柱（DE52）对嗜酸乳酸杆菌的S-层蛋白进行提纯,然后分别研究了嗜酸乳酸杆菌和S-层蛋白对鼠伤寒沙门氏菌黏附及入侵Caco-2细胞的作用。【结果】S-层蛋白能显著地抑制鼠伤寒沙门氏菌的黏附及入侵;在竞争、排斥、置换3种黏附试验中,S-层蛋白可显著降低鼠伤寒沙门氏菌的黏附,其相对黏附力分别为1.17%±5.97%、8.71%±1.36%、10.56%±0.92%,差异极显著（p〈0.01）,其中竞争试验效果最好;并且S-层蛋白对鼠伤寒沙门氏菌黏附抑制作用极显著高于嗜酸乳酸杆菌（p〈0.01）;此外,S-层蛋白也能显著抑制鼠伤寒沙门氏菌入侵。【结论】乳酸杆菌S-层蛋白对鼠伤寒沙门氏菌可产生显著的拮抗作用,这可能与S-层蛋白和鼠伤寒沙门氏菌的宿主黏附受体存在竞争作用有关;提示乳酸杆菌S-层蛋白可用于预防和治疗鼠伤寒沙门氏菌感染,并有望成为抗生素的替代品。[Objective]S-layer proteins of Lactobacillus acidophilus were extracted and purified,then the effect of Lactobacillus acidophilus and its S-layer proteins against the adhesion and invasion of salmonella typhimurium to caco-2 cells were investigated.[Methods]S-layer proteins were purified by anion-exchange column{diethylaminoethyl（DEAE） DE52},and the inhibition of Lactobacillus acidophilus and its S-layer proteins were studied against the adhesion and invasion of salmonella typhimurium on Caco-2 cells.[Results]S-layer proteins exhibited strongly inhibitory effects of adhesive and invading properties of Salmonella typhimurium.In the adhesive experiments（competitive,exclusive and displacement）,Salmonella typhimurium adhesion was reduced by S-layer proteins and the ability of adherence to Caco-2 cells were 1.17 % ± 5.97 %,8.71 % ± 1.36 % and 10.56 % ± 0.92 %,respectively（P0.01）.The influence to inhibit the competitive adhesion of Salmonella typhimurium was optimal.Furthermore,the S-layer proteins showed a stronger effect than Lactobacillus acidophilus to inhibit Salmonella typhimurium adhesion on Caco-2 monolayers（P0.01）.Moreover,invasion of Salmonella typhimurium to Caco-2 monolayers was inhibited by S-layer proteins.[Conclusion]S-layer proteins inhibited adherence and invasion of Salmonella typhimurium.The result can merit a highlight for preventive or probiotic therapy in human or animals with disease caused by Salmonella typhimurium.

关 键 词：嗜酸乳酸杆菌 S-层蛋白 鼠伤寒沙门氏菌 CACO-2细胞 

分 类 号：R371[医药卫生—病原生物学]