Ceratotropis亚属基于SSR标记和ITS序列的分类与进化分析  

Classification and Evolution Analysis for Vigna Subgenus Ceratotropis Based on SSR Markers and ITS Sequences

作　　者：马燕玲[1] 程须珍[1] 王丽侠[1] 王素华[1] 赵丹[1] 

机构地区：[1]中国农业科学院作物科学研究所,北京100081

出　　处：《作物学报》2010年第9期1585-1595,共11页Acta Agronomica Sinica

基　　金：北京市自然科学基金项目(6103025);国家食用豆产业技术体系建设项目(nycytx-18);食用豆行业科技专项(nyhyzx07-017)资助

摘　　要：利用SSR分子标记和ITS序列分析研究Ceratotropis亚属已鉴定的93份种质以及Vigna亚属的2份地方种和Glycine属的1份野生种,共8个种及亚种的96份材料的遗传差异。初步筛选出分布于11个连锁群（linkage group,LG）的74对小豆引物及14对黒吉豆引物和第9连锁群的5对绿豆引物,得到分辨力强的27对引物,平均PIC值为0.4914;UPGMA聚类分析,在DICE系数0.021处,将95份参试材料（除HAI_103）划分为两大类,第I类包括Ceratotropis组所有种质和3个＂过渡＂种质;第Ⅱ类包括Angulares组所有种质,且小豆引物通用性最高,23对小豆引物的转移能力达88%以上。对参试材料中的28份典型种质进行ITS测序分析,比较序列间Kimura进化距离发现,V.minima和V.umbellata种间距离最小（0-0.005）,V.radiata种内分化程度最高;利用MEGA4.0构建最大简约树（maximum parsimony,MP）和邻接树（neighbor-joining,NJ）,将参试种质划分为3大类,这与形态学和SSR标记分类一致。Ninety-three materials in subgenus Ceratotropis, two landraces of subgenus Vigna and one accession of Glycine soja from various regions of China were analyzed by using simple sequence repeat （SSR） makers and sequencing nrDNA internal transcribed spacer （ITS） for the genetic evaluation. Twenty-seven specific SSR primers were selected by screening 74 azuki bean primers and 14 blackgram primers that distributed in 11 linkage groups and five mungbean primers in linkage group 9 with the average polymorphic information content （PIC） value 0.4914. In UPGMA cluster analysis, 95 materials excluding HAI_103 （G. soja） were divided into Group I （including section Ceratotropis and three ＂transitional＂ materials） and Group II （section Angulares）. And the transferability of 23 primers from azuki bean reached 88%. As for ITS analysis, Kimura-2-parameter distance was calculated based on ITS sequences of 28 representing materials. The shortest distance was found between V. minima and V. umbellata with 0–0.005 and V. radiata was the most intra-specific divergent. Phylogenetic trees constructed by maximum parsimony （MP） and neighbor-joining （NJ） methods with MEGA 4.0 showed that 28 materials were divided into three groups. This result was consistent with the classification results by phenotypes and SSR makers. Therefore, it is anticipated that more information for classification of subgenus Ceratotropis can be provided by these two molecular methods and also a system for evaluation combined by morphological characteristics can be established.

关 键 词：Ceratotropis亚属 分类 SSR标记 ITS序列 进化 

分 类 号：S643.4[农业科学—蔬菜学]