细胞外信号调节激酶蛋白在全脑缺血再灌注损伤后的表达及与凋亡的关系  

The expression of ERK after global cerebralhemia/rep isc erfusion and discover the correlation between APOPTOSIS and ERK

作　　者：吴献伟[1] 杨华[1] 吴丽[1] 薛荣亮[1] 

机构地区：[1]西安市红十字会医院,710054

出　　处：《内蒙古中医药》2010年第15期103-104,共2页Inner Mongolia Journal of Traditional Chinese Medicine

摘　　要：目的:ERK蛋白的表达与细胞凋亡的关系来探讨ERK通路在脑缺血再灌注损伤中的作用。方法:健康雄性SD大鼠60只,随机分为两组,采用4-VO法建立全脑缺血再灌注模型,将脑组织切片进行HE染色、免疫组化染色和TUNEL检测。结果:IR组ERK在海马CA1区锥体细胞未见表达,CA3区于再灌注后2h有弱表达,于再灌注6h达高峰后逐渐下降,至再灌注后48h未见表达;IR组中CA1区2h即有散在的凋亡细胞,24h-48h达峰值,结论:ERK信号转导通路在脑缺血再灌注中参与了缺血后神经元的保护,发挥抗凋亡作用。Objective： To study the relationship between expression of p- ERK with cell apoptosis and cell apeptosis. Methods： 60 healthy male SD Rats were divided into 3 groups randomly, global cerebral ischemia/reperfusion model was produced by 4 - VO method. HE staining , TUNEL and Immunohistochemical staining of brain tissue section were performed. Results ： There was no p - ERK expressed in CA1 of m group. In CA3 increased after that time and peaked at 6h, then decreased . The positive straning were localized in nucelus, and the nucleus is more obviously, until 48h there was no expression. TUNEL detection in IR group showed that the number of positive cell peaked at 24h - 48h after global cerebral ischemi- a/reperfusion in CA1 of hippocampus. Conclusions： ERK expressed in both CA1 and CA3 of hippocampus after cerebral ischemia/reperfusion, which showed different intensity with different time. ERK gived play to pretect neurons and righted against apoptosis.

关 键 词：脑缺血再灌注损伤 ERK 凋亡 

分 类 号：R698.2[医药卫生—泌尿科学]