大鼠力竭游泳运动外周血内mi-422a与大脑皮质Bcl-w表达与运动性中枢疲劳  

The Relationship Between the Expression of Mir-422a in Peripheral Blood and Bcl-w in Cerebral Cortex and Exercise-Induced Central Fatigue

作　　者：杨立[1] 

机构地区：[1]西北民族大学体育学院,甘肃兰州730030

出　　处：《成都体育学院学报》2010年第9期69-71,共3页Journal of Chengdu Sport University

摘　　要：研究目的:探讨外周血内Mir-422a可否监测运动性中枢疲劳。研究方法:健康雄性Wister大鼠36只,随机分为对照组(C组)、一次性力竭运动组(E组)。力竭运动后即刻、12h和24h取各组外周血和大脑皮质分别采用real-time PCR和免疫组织化学技术检测各组外周血Mir-422a和大脑皮质Bcl-w表达。结果:力竭运动后即刻Mir-422a表达降低(P<0.05),而Bcl-w表达升高(P<0.05);12h后Mir-422a表达逐渐升高,而Bcl-w表达逐渐降低,24h均基本恢复到安静水平。结论:外周血内Mir-422a的表达有望成为运动性中枢疲劳的监测指标。Objective： To explore whether Mir-422a can monitor exercise -induced central fatigue. Methods： 36 healthy male Wister rats are randomly divided into the control group （ group C） and the exhaustive group （ group E）. The expression of Mir - 422a in peripheral blood and Bcl - w in cerebral cortex is tested by real - time PCR and immu- nohistochemistry immediately, 12 hours and 24 hours after the exhaustive exercise. Results： The expression of Mir - 422a drops while the expression of Bcl - w jumps immediately after the exercise （ P 〈 0.05 ） ; the expression of Mir - 422a gradually increases while the expression of Bcl- w gradually decreases 12 hours after the exercise; the expression of the two both regain the previous level 24 hours after the exercise. Conclusion ： The expression of Mir - 422a in the pe- ripheral blood is likely to be the index to monitor exercise -induced central fatigue.

关 键 词：运动性中枢疲劳 力竭游泳运动 Mir-422a bcl—w 

分 类 号：G804.2[文化科学—运动人体科学]