选择性急性肺血管栓塞实验模型的建立  

Establishment of selected acute pulmonary thromboembolism model in experimental sheep

作　　者：樊济海[1] 顾秀莲[1] 巢胜吾[1] 张鹏[1] 潘瑞麟[1] 王丽娜[1] 王璐璐[1] 王玲[1] 李博[1] 陈涛涛[1] 

机构地区：[1]中国人民解放军第四五五医院心内科,上海200052

出　　处：《介入放射学杂志》2010年第8期639-641,共3页Journal of Interventional Radiology

基　　金：上海市长宁区卫生局局级课题(编号:20074Y16001)

摘　　要：目的建立绵羊急性肺血栓栓塞(APTE)模型。方法实验用绵羊10只,采用Seldinger血管穿刺技术,建立股静脉和股动脉导管通路,在C型臂数字减影X线透视下,将导管插入肺动脉,将羊自体血栓注入选择的肺动脉分支,监测肺动脉造影,肺动脉压力、股动脉压力、心率、PaO2等指标评估肺栓塞模型的建立。结果基础肺动脉压力(27.3±9.6)mmHg,股动脉收缩压(126.4±13.7)mmHg,心率(103±15)次/min,股动脉血PaO2(87.7±12.0)mmHg,n=10;注入血栓剂量(30±5)ml后60min,肺动脉压力(52±49)mmHg;股动脉收缩压(100±21)mmHg;心率(150±26)次/min;股动脉血PaO2(62.4±11.1)mmHg,P均<0.01;肺动脉造影显示选择的肺动脉栓塞成功。结论实验绵羊股静脉、腔静脉系统及右心系统解剖学特征适合于建立导管通路,建立选择性急性肺栓塞模型;建立选择性绵羊急性肺栓塞实验模型可行性、稳定性良好,具有栓塞部位、范围、严重程度的可控制性,影像学特征更适合于急性肺栓塞的实验研究。Objective To establish a selected acute pulmonary thromboembolism model in experimental sheep suitable for animal experiment. Methods By using Seldinger＇s technique the catheter sheath was placed in both the femoral vein and femoral artery in ten sheep. Under C-arm DSA guidance the catheter was inserted through the catheter sheath into the pulmonary artery. Via the catheter appropriate amount of sheep autologous blood clots was injected into the selected pulmonary arteries. The selected acute pulmonary thromboembolism model was thus established. Pulmonary angiography was performed to check the results. The pulmonary arterial pressure,femoral artery pressure,heart rates and partial pressure of oxygen in arterial blood （PaO2） were determined both before and after the treatment. The above parameters obtained after the procedure were compared with the recorded parameters measured before the procedure,and the sheep model quality was evaluated. Results The baseline of pulmonary arterial pressure was （27.30 ± 9.58） mmHg,femoral artery pressure was （126.4 ± 13.72） mmHg,heart rate was （103 ± 15） bpm and PaO2 was （87.7 ± 12.04） mmHg. Sixty minutes after the injection of （30 ± 5） ml thrombotic agglomerates,the pulmonary arterial pressures rose to （52 ± 49） mmHg,femoral artery pressures dropped to （100 ± 21） mmHg. The heart rates went up to （150 ± 26） bpm. The PaO2 fell to （25.3 ± 11.2） mmHg. After the procedure the above parameters were significantly different from that measured before the procedure in all ten animals （P 〈0.01）. The pulmonary arteriography clearly demonstrated that the selected pulmonary arteries were successfully embolized. Conclusion The anatomy of sheep＇s femoral veins,vena cava system,pulmonary artery and right heart system are suitable for the establishment of the catheter passage,for this reason,selected acute pulmonary thromboembolism model can be easily created in experimental sheep. The technique is feasible and the model has the advantage

关 键 词：肺血栓栓塞症 肺栓塞 动物模型 导管插入术 绵羊 

分 类 号：R563.5[医药卫生—呼吸系统]