胃溃疡大鼠在合募配穴针刺后胃组织的纳升级二维液相色谱  

Nano-2D-LC analysis of proteomic alterations in rats with gastric stress ulcer after acupuncture at He-Sea point and Front-Mu point of the stomach meridian

作　　者：杨波[1] 严兴科[2] 

机构地区：[1]长春中医药大学研究生学院,吉林省长春市130117 [2]长春中医药大学针灸推拿学院,吉林省长春市130117

出　　处：《世界华人消化杂志》2010年第22期2355-2358,共4页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30873310;2009年度教育部高等学校博士学科点专项科研基金资助项目;No.20092227120002;吉林省教育厅"十一五"科学研究基金资助项目;No.2009-377~~

摘　　要：目的:揭示合募配穴法治疗胃溃疡特异性效应和协同机制.方法:大鼠35只随机分为空白对照组、应激性胃溃疡模型组、募穴组(中脘穴)、下合穴组(足三里组)和合募配穴组(中脘穴配足三里穴组),利用水浸加捆绑法复制应激性胃溃疡模型大鼠,借助纳升级二维液相色谱(Nano-2D-LC)技术研究大鼠胃经下合穴及募穴针刺后胃组织差异蛋白表达谱峰.结果:在保留时间27和32min时,模型组、募穴组、下合穴组和合募配穴组比空白对照组多出色谱峰5个,并且色谱峰5个的峰面积依次对应缩小.结论:针刺对应激性胃溃疡的治疗机制可能与5种多肽含量的减小有关.AIM：To investigate the therapeutic effect of acupuncture at He-Sea point and Front-Mu point of the stomach meridian on gastric stress ulcer（SU） in rats and to explore potential mechanisms involved.METHODS：Thirty-five Wistar rats were randomly divided into five groups：blank control group,model group,He-Sea point（Zusanli） group,Front-Mu point（Zhongwan） group,and He-Sea and Front-Mu point（He-Mu） group.Gastric SU was induced in rats by water immersion and restraint.Proteomic alterations in the stomach of rats were analyzed by nano-twodimensional liquid chromatography（nano-2DLC）.RESULTS：Five chromatographic peaks at a retention time between 27 and 32 min were noted on the chromatograms in the model group and acupuncture treatment groups.The retention time of these five peaks was basically identical.The peak area was largest in the model group,followed by the Front-Mu point group,He-Sea point group and He-Mu group.CONCLUSION：Acupuncture at He-Sea point and Front-Mu point exerts a therapeutic effect on SU possibly by altering the expression of some proteins.

关 键 词：应激性胃溃疡 合募配穴 纳升级二维液相色谱 差异多肽谱峰 

分 类 号：R245[医药卫生—针灸推拿学]