流场压力变化对体外培养内皮细胞合成与表达功能蛋白的影响  

Pressure Change-mediated Effects on Vasoactive Protein of Endothelial Cells in the Flow Field in Vitro

作　　者：胡佳[1] 张本贵[1] 吴江[2] 张尔永[1] 许唯临[3] 石应康[1] 郭应强[1,3] 

机构地区：[1]四川大学华西医院胸心血管外科,成都610041 [2]四川大学华西基础医学与法医学院生物医学工程研究室,成都610041 [3]四川大学高速水力学国家重点实验室,成都610044

出　　处：《生物医学工程学杂志》2010年第4期795-800,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30700149;30670515;10802054);教育部博士点基金资助项目(20050610060;20070610158)

摘　　要：内皮细胞(ECs)衬覆于血管内腔面,所构成的选择性生理屏障进行血管内外物质交换而保证了血管结构和功能的正常,ECs通过分泌多种生物活性因子来调节局部血管流场压力,以适应生理活动的变化。本研究采用改良流动培养的流室压力系统对ECs加载不同压力,同时设置静态培养、单纯剪应力对照组,结合ELISA法和定量RT-PCR技术,检测ECs内皮素-1(ET-1)、内皮型一氧化氮合成酶(eNOS)、环氧化酶-2(COX-2)及内皮细胞生长因子(VEGF)等功能蛋白分子的变化情况,从而观察流场内不同压力信号(特别是压力降信号)是否会引起ECs功能蛋白表达与合成异常,分析其量-效关系变化,以便进一步了解不同压力信号诱发内皮细胞功能障碍的机理。研究结果表明,ECs可以通过调节功能蛋白分子的表达来适应局部流场内压力信号的变化,而在流场压力降信号(-40 cmH2O)诱导的内皮细胞功能障碍过程中,eNOS、COX-2和VEGF在一定范围内起着保护ECs完整性的重要作用。Lining the inner surface of the walls of blood vessels,Endothelial cells（ECs） go beyond providing selective membrane to maintain the natural structure and function of vessels;they also synthesize varieties of vasoactive proteins to modify the pressure shift in the local flow field and hence they adapt the physiological activities of vessels.In this experiment,ELISA and RT-PCR technologies were adopted.We set up five different pressure loaded ECs groups,one non-activated cultured ECs group and one single shear stress loaded ECs group.Such a design was intended to demonstrate the effects of pressure shift on the expression of vasoactive protein synthesized by ECs [Endothelin-1（ET-1）,endothelial Nitric Oxide Synthase（eNOS）,Cyclooxygenase-2（COX-2） and Vascular Endothelial Growth Factor（VEGF）].Our aim was to elucidate the mechanism of the pressure shift mediated dysfunction in ECs and the related dose-effect relationship.Based on these data,we suggest that ECs could modify the expression of vasoactive protein for adapting to the pressure shift in the local flow field;while in the process of-40cmH2O induced ECs＇ dysfunction,the vasoactive proteins eNOS,COX-2 and VEGF play an important role in protecting ECs.

关 键 词：内皮细胞 流场压力 功能蛋白 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R318.01[医药卫生—基础医学]