阿胶对成纤维细胞在丝素材料表面黏附增殖的促进作用  

The Surface Modification of Eguus Asinus Augment Fibroblast Adhesion And Proliferation on Silk Fibroin Materials

作　　者：连小洁[1] 王松[1,2] 朱鹤孙[2] 高珍[1] 

机构地区：[1]北京理工大学材料科学与工程学院,北京100081 [2]北京理工大学材料科学研究中心,北京100081

出　　处：《生物医学工程学杂志》2010年第4期806-810,共5页Journal of Biomedical Engineering

基　　金：国家重点基础研究"973"计划项目资助(2005CB623902)

摘　　要：利用中药阿胶对丝素材料改性,制备一系列不同阿胶含量的阿胶改性丝素材料,在材料表面种植鼠成纤维细胞NIH-3T3,用光学显微镜跟踪拍摄材料表面细胞形态变化,用微吸管法测试细胞黏着直径和初始黏附力,用MTT比色法评价其细胞增殖能力。结果发现,成纤维细胞在阿胶改性丝素材料表面比纯丝素材料具有更强的初始黏附力、更大的细胞黏着面积和更高的细胞增殖能力,表明阿胶能够促进成纤维细胞在改性丝素材料表面的黏附和增殖。这一研究有利于拓展中药阿胶在组织工程材料领域的应用。Eguus asinus is one of the rare Chinese drugs famous for promoting blood circulation.In this experiment,it was employed to modify the silk fibroin（SF） by physical blending.Mouse embryonic fibroblasts NIH-3T3 were seeded on pure and modified SF surfaces.The morphological changes of cell on SF surfaces were characterized by optical microscope images.The cell adhesion diameter,the attachment force（F） of a single cell and cell proliferation on SF surfaces were measured by micropipette aspiration system and MTT assay.The results indicated that the fibroblasts seeded on modified SF had larger cell adhesion area,stronger initial attachment force and higher cell proliferation than did the pure SF.In conclusion,eguus asinus improved the adhesion and proliferation of fibroblasts on SF.The research promoted the further use of Chinese drug eguus asinus in tissue engineering.

关 键 词：阿胶 丝素蛋白 成纤维细胞 微吸管 MTT比色法 

分 类 号：R318.08[医药卫生—生物医学工程]