去甲肾上腺素和乙酰胆碱对大鼠离体室旁核神经元放电活动的影响  

EFFECTS OF NORADRENALINE AND ACETYLCHOLINE ON ELECTRIC ACTIVITY OF PARAVENTRICULAR NEURONS OF RAT HYPOTHALAMIC SLICES

作　　者：邢宝仁[1] 郭益民[2] 夏金辉[1] 陈宜张[1] 

机构地区：[1]第二军医大学生理学教研室神经科学实验室 [2]温州医学院生理学教研室

出　　处：《生理学报》1990年第5期413-419,共7页Acta Physiologica Sinica

基　　金：自然科学基金;总后协作攻关课题资金

摘　　要：用31个脑片观察了去甲肾上腺素(NA)和乙酰胆碱(ACh)对90个室旁核神经元放电活动的影响。脑片灌流 NA(10^(-6)mol/L,3min)后,14/78(17%)个非周期型放电单位和7/12(58.3%)个周期型放电单位的放电频率增加,10/78(12%)个非周期型放电单位和2/12(16.6%)个周期型放电单位的放电频率明显降低甚至完全停止,54/78(69%)非周期型单位和3/12(25%)个周期型单位无明显反应。NA 对非周期型放电单位的兴奋作用可被 α 受体阻断剂酚妥拉明完全阻断,而 NA 对周期型单位的兴奋作用只能被部分阻断。脑片灌流 ACh(10^(-7)mol/L,3min)后,使15/78(19%)个非周期型和6/12(50%)个周期型单位放电频率增加,9/78(11%)非周期型和2/12(16.6%)个周期型放电单位放电频率降低,54/78(69%)非周期型和4/12(33.3%)周期型单位无反应。阿托品或东莨菪碱可翻转 ACh 对它们的作用。实验提示,NA 和 ACh 对室旁核神经元的兴奋或抑制作用是分别由 α、β 和 Μ 受体介导的。The effects of noradrenaline(NA)and acetylcholine(ACh)on 90 paraventri-cular neurons from 31 hypothalamic slices prepared from rat were observed.Afterperfusing the brain slices with NA(10_(-6)mol/L,3 min),discharge rate of 14/73(17%)nonphasic and 7/12(58.3%)phasic neurons was significantly increased,while that of 10/73(12%)nonphasic and 2/12(16.6%)phasic neurons was sig-nificantly decreased,even ceased.50/73(64%)of the nonphasic and 3/12(25%)of the phasic neurons were non-responsive.The effects of NA on the nonphasicneurons were completely blocked by α receptor blocker phentolamine.The effectsof NA on phasic neurons were partly blocked by phentolamine or propranolol(βreceptor blocker).After perfusing hypothalamic slices with ACh(10^(-7) mol/L,3min),the firing rate of 15/73(19%)nonphasic and 6/12(50%)phasic neurons wasincreased,while that of 9/73(11%)nonphasic and 2/12(16.6%)phasic neuronswas decreased.54/73(69%)nonphasic and 4/12(33.3%)phasic neurons werenon-responsive,The effect of ACh on the neurons could be reversed by atropine.The effect of scoplamine(M blocker)is similar to that of atropine.The experi-mental results suggest that the excitatory or inhibitory effect of NA or ACh on para-ventricular neurons has mediated respectively by α,βor M receptors.

关 键 词：室旁核神经元 放电活动 NA ACH 

分 类 号：R338.8[医药卫生—人体生理学]