溶藻弧菌脂多糖对大菱鲆免疫保护实验  

Vibrio alginolyticus lipopolysaccharide on turbot immune protection test

作　　者：张伟[1] 叶海斌[1] 朱安成 李天保[1] 

机构地区：[1]山东省海水养殖研究所,山东省海水养殖病害防治重点实验室,山东青岛266002

出　　处：《水产养殖》2010年第9期18-19,共2页Journal of Aquaculture

基　　金：山东省科技厅(2004GG2205135)

摘　　要：采用粗提脂多糖（LPS）对体重为110~150g大菱鲆进行免疫保护实验。注射浓度为20mg/mL和40mg/mL的LPS溶液0.2mL,以及按5‰比例添加到饲料中进行投喂试验。免疫后用0.1mL浓度为1×109CFU/mL的溶藻胶弧菌分别进行5次感染实验。结果显示：第7天即可产生免疫保护作用,RPS可达75%,免疫保护作用可持续3个月。Using crude lipopolysaccharide（LPS）on body weight of 110～150 g turbot for protective immunization. Injection concentration 20 mg/mL and 40 mg/mL of solution 0.2 mL LPS,and the ratio by 5 ‰ to the feed for feeding test. 0.1 mL after immunization with the concentration of 1×109CFU/mL of Vibrio alginolyticus gum infection experiments were carried out 5 times. The results showed that,7 days to generate immune protection,RPS 75%,and immune protection sustainable for 3 months.

关 键 词：溶藻弧菌 细菌脂多糖 大菱鲆 免疫保护率 

分 类 号：S943[农业科学—水产养殖]