皮质酮对大鼠下丘脑薄片室旁核神经元自发电活动的影响  

EFFECTS OF CORTICOSTERONE ON SPONTANEOUS DISCHARGES OF RAT HYPOTHALAMIC PARAVENTRICULAR NEURONS IN VITRO

作　　者：顾勤[1] 邢宝仁[1] 夏金辉[1] 陈宜张[1] 

机构地区：[1]第二军医大学生理教研室

出　　处：《生理学报》1990年第5期476-482,共7页Acta Physiologica Sinica

摘　　要：本实验应用离体大鼠下丘脑薄片技术,用玻璃微电极细胞外记录、观察了下丘脑室旁核神经元的自发电活动,以及皮质酮对自发电活动的影响。在36个下丘脑薄片上观察了104个室旁核神经元的自发电活动,其放电形式主要有三种:慢而不规则(50个,占48.1%)、快速连续(44个,占42.5%)和周期性放电(10个,9.4%)。在进行皮质酮灌流的实验中,这104个单位有25个在皮质酮(10^(-7),10^(-6)mol/L)作用后,自发放电明显减少,有8个单位出现兴奋效应;其余的没有观察到明显反应。上述33个产生反应的神经元其反应特点是:潜伏期短、反应的程度与皮质酮浓度有关、糖皮质激素胞液受体阻断剂 RU38486可以阻断这种反应。结果表明糖皮质激素可以快速影响下丘脑薄片内某些室旁核神经元的电活动,为甾体激素的快速非基因机制作用提供了新的证据,提示室旁核神经元膜上有糖皮质激素受体存在。Experiments were performed to investigate the effects of corticosterone on ele-trical activities of paraventricular neurons in rat hypothalamic slices.In unstimu-lated conditions,most of the neurons had either a slow irregular(48.1%)or fastcontinuous pattern(42.5%)of discharge.Only a small portion(9.4%)of the cellsshows phasic activity.During perfusion of the slices with corticosterone(10^(-7),10^(-6)mol/L),25 out of 104 paraventricular neurons were profoundly inhibited with ashort latency,8 neurons were excited and the remaining cells showed no response.The response,the magnitude of the cells which depended upon the concentration ofcorticosterone,could be reversed by concomitant application of RU38486(10^(-7),10^(-6)mol/L),a competitive antagonist of glucocorticoid's cytosolic receptor.These results provide further evidence that corticosterone has rapid effects onrat hypothalamic paraventricular neurons,and suggests the existence of membranereceptors for glucocorticoid in paraventricular neurons.

关 键 词：下丘脑室旁核 皮质酮 神经元放电 

分 类 号：R338.8[医药卫生—人体生理学]