三七总皂苷对缺氧再给氧血管内皮细胞NF-κB及ICAM-1表达的影响  

Effect of Total Panax Notoginseng Saponins on Nuclear Factor Kappa-B and Intercellular Adhesion Molecule-1 of ECV304 in Hypoxia/reoxygenation InjuryJiang

作　　者：江小萍 李海全 潘少霞 方永奇[2] 刘桂娥 欧阳永红 沈祖泓 

机构地区：[1]广东省清远市中医院 [2]广州中医药大学第一临床学院

出　　处：《中西医结合心脑血管病杂志》2010年第9期1086-1088,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：广东省中医药局建设中医药强省科研基金资助项目(No.2007292)

摘　　要：目的探讨人脐静脉内皮细胞株(ECV304)缺氧再给氧(H/R)损伤的发生机制及三七总皂苷(tPNS)对该病理生理过程的影响。方法将体外培养的ECV304分为七组,正常组;模型组;tPNS高、中、低浓度组;维拉帕米组;吡咯烷二硫氨基甲酸酯(PDTC)组。对各组进行相应处理后,分别检测各组ECV304超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、丙二醛(MDA)的含量、核转录因子-B(NF-κB)及细胞间黏附分子-1(ICAM-1)的表达。结果模型组,tPNS高,中、低浓度组、维拉帕米组及PDTC组细胞氧自由基清除功能明显低于正常组,NF-κB及ICAM-1表达明显高于正常组,其中tPNS高、中、低浓度组细胞氧自由基清除功能明显高于H/R组,NF-κB及ICAM-1表达率明显低于H/R组,且呈浓度依赖性改变,tPNS高浓度组细胞氧自由基清除功能明显高于维拉帕米组及PDTC组,NF-κB及ICAM-1表达率明显低于维拉帕米组及PDTC组同期。结论 H/R可激活ECV304,使细胞氧自由基清除功能明显降低,NF-κB及ICAM-1的表达明显升高;tPNS能有效改善细胞氧自由基清除功能,降低NF-κB、ICAM-1活性,减轻H/R损伤,且高浓度tPNS效果优于维拉帕米、PDTC。Objective To study the mechanisms of ECV304 with hypoxia/reoxygenation（H/R） injury and the effect of total panax notoginseng saponins（tPNS） on the pathophysiologic process.Methods The cultured ECV304 were divided into 7 groups：Control group,H/R group,tPNS group（high,middle and low concentrations）,verapamil group,pyrrolidine dithiocarbamate（PDTC） group.The contents of superoxide dismutase（SOD）,glutathione（GSH）and malondialdehyde（MDA）in ECV304 were measured.The nuclear factor kappa-B（NF-κB）and intercellular adhesion molecule-1（ICAM-1）expression of ECV304 were detected.Results The content of SOD and GSH in H/R group,tPNS group,verapamil group,and PDTC group were significantly lower than that in control group.The levels of MDA,NF-κB and ICAM-1 in H/R group,tPNS group,verapamil group,and PDTC group were significantly higher than that in control group.The content of SOD and GSH in tPNS group were significantly higher and the levels of MDA,NF-κB and ICAM-1 were significantly lower than that in H/R group.The higher concentration of tPNS was more effective.The GSH in high concentrations of tPNS group was significantly higher than that in verapamil group and PDTC group.The levels of MDA,NF-κB and ICAM-1was significantly lower than that in verapamil group and PDTC group.Conclusion The results suggested that H/R could activate ECV304,increase the expression of MDA,NF-κB and ICAM-1.The tPNS could inhibit the expression of MDA,NF-κB and ICAM-1 efficiently and alleviate H/R injury.The high concentration of tPNS was more efficiently than verapamil and PDTC.

关 键 词：人脐静脉内皮细胞株 缺氧再给氧损伤 核转录因子 细胞间黏附分子-1 三七总皂苷 

分 类 号：R285.5[医药卫生—中药学]