BTH诱导不同抗性水平甜瓜抗白粉病的效应  

Induction of resistance of melon cultivars with different resistances to powdery mildew after BTH treatment

作　　者：陈年来[1,2] 朱振家[1] 代春艳[2] 王春林[1] 安翠香[1] 

机构地区：[1]甘肃农业大学农学院 [2]甘肃农业大学资源与环境学院,兰州730070

出　　处：《果树学报》2010年第5期764-769,共6页Journal of Fruit Science

基　　金：甘肃省农业生物技术研究与开发应用(编号:GNSW-2006-08)

摘　　要：诱导植物内在抗性是病害控制的新策略,诱导效应可能与不同品种组成性表达的基础抗性有关。以3个抗性水平不同的品种为材料,研究了苯并噻二唑(BTH)对甜瓜白粉病抗性的诱导效应,以期进一步阐释抗性诱导效应与品种基础抗性的关系。结果显示,BTH诱导甜瓜抗白粉病的效果与品种的基础抗性正相关,对抗病品种的诱导效果高于中抗和感病品种,抗病品种叶片中β-1,3-葡聚糖酶(GLU)和苯丙氨酸解氨酶(PAL)活性增长幅度也较中抗和感病品种大。BTH处理3 d后接种白粉菌,不同抗性水平甜瓜品种叶片中GLU、PAL和多酚氧化酶(PPO)活性均较只接种处理明显上升。叶片超微结构观察显示:乳突结构是寄主对病原菌侵染的一种主动反应,甜瓜抗病品种叶片中乳突结构多呈半圆球型,中抗和感病品种叶片中多呈圆筒型。Induction of potential resistance in plants is a new strategy of disease control. Three melon cultivars with different basic resistance levels were employed to evaluate the effects of BTH spaying on induced resistance to powdery mildew, attempting to understand further the relationship between induced and constitutive resistance. The results showed that positive correlations existed between the induction effect and the intrinsic resistance to powdery mildew of melon cultivars. The resistant cultivars showed higher induced resistance to powdery mildew and had higher increase rate in the activity of GLU and PAL in leaf than tolerant and susceptible cultivars. The activities of GLU,PAL and PPO in leaf were higher in melon plants treated with BTH and then inoculated with Erysiphe cichoracearum than those in the plants only inoculated. Ultrastructure observation showed that papilla might be an active responsive part of melon leaf to Erysiphe cichoracearum.The papilla structure in the resistant cultivar was hemispherical, but cylinder in the tolerant and susceptible cuhivars.

关 键 词：甜瓜 BTH 白粉病 抗性酶活性 超微结构 

分 类 号：S652[农业科学—果树学]