单细胞凝胶电泳法检测HBV患者外周血淋巴细胞DNA损伤  

Detection of DNA damage in peripheral blood lymphocytes of HBV-infected patients with single-cell gel electrophoresis

作　　者：黄金梅[1] 曾小云[1] 仇小强[2] 

机构地区：[1]广西医科大学公共卫生学院流行病学与卫生统计学教研室,南宁530021 [2]桂林医学院,广西540021

出　　处：《中国临床新医学》2010年第8期697-700,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金项目(No.30860247);广西自然科学基金项目(桂科青0728066)

摘　　要：目的探讨乙型肝炎病毒与DNA损伤的关系以及不同感染模式慢性乙型肝炎患者(大三阳和小三阳患者)DNA损伤情况。方法应用单细胞凝胶电泳(SCGE)技术检测对照组和不同感染模式慢性乙型肝炎患者外周血淋巴细胞DNA的损伤情况,采用彗星分析软件对实验结果进行淋巴细胞损伤率、彗星尾长(Tail Extent)、彗星尾DNA百分含量(Tail % DNA)、彗星尾惯量(Tail Inertia)、Olive尾距(Olive Tail Moment)等指标进行分析。结果经SCGE技术后得HBV感染与DNA损伤断裂关系有统计学意义(P<0.05);大小三阳组间DNA损伤断裂有统计学差异(P<0.05)。小三阳组经SCGE技术后所得彗星图像可见有少量彗星尾。大三阳组经SCGE技术后所得彗星图像可见有明显彗星尾。慢性乙型肝炎组外周血淋巴细胞DNA损伤与对照组比较彗星尾长、尾惯量在统计学上有显著性差异(P<0.05);不同感染模式的乙型肝炎外周血淋巴细胞DNA损伤比较,尾长、尾DNA百分含量和尾惯量在统计学上有统计学差异(P<0.05)。结论 HBV感染可能造成外周血淋巴细胞DNA损伤,且不同感染模式的损伤情况不同。Objective To explore the relationship between the hepatitis B virus and DNA damage, and explore DNA damage situation around the different mode of infection of HBV-infected patients. Methods Single-cell gel electrophoresis （SCGE） technique was adopted in measuring DNA damage in peripheral blood lymphocytes of controls, different models of chronic HBV-infeeted patients and the comet analysis software was used for analyzing the results. The degree of DNA damage was judged by the comet tail length （tail extent） , the percentage of the comet tail DNA （Tail% DNA）, the comet tail of inertia （tail inertia） , olive tail moment. Results There were significantly differences between infection of HBV and DNA damage （P 〈 0. 05 ） , and also significantly differences between the dif- ferent mode of infection of HBV-infeeted patients （ P 〈 0. 05） after the SCGE . There were a few comet tail images in lymphocytes of xiaosanyang group and obviously comet tail images in lymphocytes of dasanyang group . The tail extent , tail % DNA in lymphocytes of chronic hepatitis B patients were aggravated significantly than in lymphocytes of controls （ P 〈 0. 05 ） , and there were significantly differences between the tail extent , tail % DNA, tail inertia of the different mode of infection in HBV-infected patients （ P 〈 0. 05 ）. Conclusion Hepatitis B virus is likely to be the main cause of resulting DNA damage in peripheral blood lymphocytes. And different mode of infection of HBV-infeeted patients have the different damage situation.

关 键 词：单细胞凝胶电泳 乙型肝炎病毒 DNA损伤 

分 类 号：R512.6[医药卫生—内科学]