高效液相色谱法测定丹参类药材中四种脂溶性成分含量  

Determination of the contents of four fat-soluble components in Salvia miltiorrhiza by HPLC

作　　者：杨树声 宋平顺[2] 赵建邦[2] 丁永辉 

机构地区：[1]定西市人民医院药剂科,甘肃定西743000 [2]甘肃省药品检验所,甘肃兰州730000 [3]甘肃省食品药品监督管理局,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2010年第3期26-28,共3页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的建立高效液相色谱法同时测定丹参、甘肃丹参中丹参酮ⅡA、丹参酮Ⅰ、二氢丹参酮和隐丹参酮含量的方法,评价药材质量。方法色谱柱:C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相:V(甲醇):V(水)=80:20;流速:1.0mL/min。结果丹参酮ⅡA、丹参酮Ⅰ、二氢丹参酮和隐丹参酮分别在17～200、10～124、11～134 ng和4～49 ng范围内线性关系良好,平均回收率分别为101.91%、98.83%、103.10%、99.95%。结论方法简便、准确、分离效果好,可作为丹参类药材含量的定量方法。Objective To establish a method to simultaneously determine the contents of tanshinone Ⅱ A, tanshinone Ⅰ , dihydro-tanshinone and cryptotanshinone in Salvia miltiorrhiza and Salvia przewalskii Maxim by high performance liquid chromatography （HPLC）. Methods Column： C18 column （4.6 mm×250mm, 5 um）; mobile phase： V （methanol） ： V（water）=80 ： 20; flow rate： 1.0 mL/min. Results Tanshinone Ⅱ A, tanshinone Ⅰ , dihydro-tanshinone and cryptotanshinone were at the range of 17-200, 10-124, 11-134 ng and 4-49 ng, respectively. The linear relation was good, and the average recovery rate was 101.91%, 98.83%, 103.10% and 99.95%, respectively. Conclusion The method is simple, accurate and effective, and can be used to determine the contents of Salvia przewalskii Maxim by HPLC.

关 键 词：丹参酮ⅡA 丹参酮Ⅰ 二氢丹参酮 隐丹参酮 丹参 甘肃丹参 

分 类 号：R927.2[医药卫生—药学]