免疫介导再生障碍性贫血小鼠模型外周血单个核细胞及脾脏干扰素-γ的表达  

Expression of IFN-γ in peripheral blood mononuclear cells and spleen of aplastic anemia mice

作　　者：侯素敏[1] 张丽春[1] 张伟华[1] 张秀莲[1] 

机构地区：[1]山西医科大学第一医院血液科,太原030001

出　　处：《中国药物与临床》2010年第9期997-999,共3页Chinese Remedies & Clinics

基　　金：山西省科技攻关项目(20090311058-5)

摘　　要：目的研究免疫介导再生障碍性贫血(AA)小鼠模型外周血单个核细胞(PBMC)及脾脏干扰素-γ(IFN-γ)表达,探讨免疫介导AA小鼠模型的免疫发病机制。方法①采用免疫介导方法建立(60Co-γ射线照射后输入淋巴细胞)AA小鼠模型。②用反转录-聚合酶链反应(RT-PCR)法检测AA小鼠PBMC中IFN-γmRNA的表达,对结果进行半定量分析。③用免疫组织化学方法检测AA小鼠脾脏组织石蜡切片中IFN-γ蛋白表达水平,以每个视野内的阳性细胞数量作为IFN-γ蛋白的量化指标,进行阳性细胞率分析。结果①AA小鼠外周血常规及网织红细胞检测显示全血细胞减少,骨髓活检显示骨髓造血细胞明显减少,非造血细胞增多。造模成功。②RT-PCR结果显示AA小鼠PBMC中IFN-γmRNA表达(1.14±0.06)高于正常小鼠(0.73±0.11),P<0.05。③免疫组织化学结果显示AA小鼠脾脏组织中IFN-γ蛋白阳性率(4.55±0.60)%高于正常小鼠(0.82±0.25)%,P<0.01。结论 AA小鼠PBMC中IFN-γmRNA和脾脏组织中IFN-γ蛋白表达水平增高,提示IFN-γ可能参与AA动物模型免疫发病机制。Objective To explore the expression of IFN-γ in peripheral blood mononuclear cells （PBMC） and spleen of aplastic anemia mice, find the role of IFN-γ in immune pathogenesis of immune-mediated aplastic anemia mice. Methods （1）Immunologically mediated method was used to imitate the model of aplastic anemia in BALB/C mice. （2）The mRNA expression of IFN-γ in PBMC of the model of aplastic anemia in BALB/C mice was measured by RT-PCR. （3）Protein expressions of IFN-γ in spleen of aplastic anemia mice model were detected by immune histochemistry. Results （1）The mRNA expression of IFN-γ in peripheral blood mononuclear ceils in aplastic anemia group was higher than that in the normal control group （1.14±0.06 vs 0.73±0.11, P〈0.05）. （2）The rate of protein expression of IFN-γ in spleen of aplastic anemia group was（4.55±0.60）%,the normal group was （0.82±0.25）%,aplastic anemia group was significantly higher than normal control group （P〈0.01）. Conclusion IFN-γ participate in the immune pathogene- sis of aplastic anemia model.

关 键 词：贫血 再生障碍性 免疫 小鼠 干扰素Ⅱ型 

分 类 号：R556[医药卫生—血液循环系统疾病]