细胞显微荧光自动检测系统的研究  

A practical system for automatic measurement of cellular microfluorescence

作　　者：范世福[1,2] 张思祥[1,2] 肖松山[1,2] 李彦芳[1,2] 王天佑[1,2] 

机构地区：[1]天津大学生物医学工程与科学仪器系 [2]北京市神经外科研究所

出　　处：《中华医学检验杂志》1999年第3期161-163,共3页

基　　金：卫生部科研基金

摘　　要：目的用普通荧光显微镜开发价廉实用、便于推广应用的细胞显微荧光自动检测系统。方法根据细胞荧光信息的生物学特点，综合运用现代光学、光电检测技术、微电子技术和计算机技术，在普通荧光显微镜上实现细胞荧光的快速、准确自动检测；根据生物学研究和临床要求，设计细胞显微荧光检测应用软件。结果在不破坏普通荧光显微镜结构的前提下，设计研制成全套细胞显微荧光自动检测系统。系统本底值在４～５小时内稳定性良好；对１２个小鼠大脑皮层神经细胞测定得静息钙离子浓度为（１４８±９２）ｎｍｏｌ／Ｌ（ｘ±ｓ）；高Ｋ＋引起Ｃａ２＋信号立即增强，Ｇｌｕ灌流后Ｃａ２＋信号的变化也立即可以明显检测出来。结论本项研究工作适应细胞分析检测的现代化要求，价廉实用便于推广应用。Objective To develop a cheap, practical and convenient measuring system for cellular microfluorescence with popular fluorescent microscope. Method According to the biological characteristics of cellular fluorescence in formation with comprehensive use of modern optics, photoelectronic measuring technique, microelectronics and computer technique, a rapid, accurate automatic measuring system for cellular fluorescence based on popular fluorescent microscope was realized. In accordance with the requests of the biological research and clinical work, a series of application software have been designed. Results Based on nondestructiveness of popular fluorescent microscope, a whole system for automatic measurment of cellular microfluorescence was designed and developed. The baseline was stable in 45 hours; the value of intracellular resting Ca2+]i in cultured mouse cerebral cortical cells was (14892) nmol/L ( s), the kinetic changes of i induced by high K+ or glutamate were traced obviously and immediately. Conclusion The system is adequate to the modernization for cellular analysis in China. It is cheap, practical and convenient.

关 键 词：显微镜 细胞学诊断 荧光自动检测 

分 类 号：R446.8[医药卫生—诊断学]