甲状腺功能低下对大鼠肝细胞核T_3受体的影响  

Effects of hypothyroidism on liver nuclear T 3 receptors

作　　者：肖建英[1,2] 刘用璋[1,2] 朱燕凌[1,2] 高淑贤[1,2] 王翠瑶[1,2] 马克玲[1,2] 

机构地区：[1]锦州医学院生化教研室 [2]锦州医学院病理生理教研室

出　　处：《中国地方病学杂志》1999年第3期192-194,共3页Chinese Jouranl of Endemiology

基　　金：辽宁省教委资助

摘　　要：目的探讨甲低大鼠肝核Ｔ３受体特性的变化，为阐明甲状腺激素对肝细胞核Ｔ３受体的作用提供实验资料。方法用０．５％ＮａＣｌＯ４诱导大鼠甲状腺功能低下，采用该室建立起来的Ｔ３和离体肝细胞核结合实验的方法，观察甲状腺功能低下对大鼠肝核Ｔ３受体的影响。结果甲状腺功能低下大鼠肝核Ｔ３受体的亲合常数（Ｋａ）增加，最大结合容量（ＭＢＣ）明显下降，Ｔ３受体占位率与对照组无显著差别，且ＭＢＣ与血清Ｔ３、Ｔ４呈正相关。结论肝核Ｔ３受体的数目受甲状腺激素的调节与控制，甲状腺激素对肝脏的作用主要通过调节核Ｔ３受体的结合容量。Objective To study the changes in character of liver nuclear T 3 receptors in hypothyroid rats and give experimental evidences to illustrate the effects of thyroid hormone on the liver nuclear T 3 receptors. Methods Hypothyroid function of rats was induced by a five week treatment with NaClO 4 and changes in the liver nuclear T 3 receptors were observed by the method of T 3 binding to isolated liver cell nuclei.Results The affinity constant (Ka) of liver nuclear T 3 receptors increases in the hypothyroid rats whereas the maximal binding capacity (MBC) decreases,and the decreases of MBC was directly related to serum T 3,T 4 concentration.It is in the hypothyroid rats that the occupancy of T 3 receptors does not change.Conclusions The numbers of nuclear T 3 receptors is under control of the thyroid hormone,and thyroid hormone acts by regulating the MBC of nuclear T 3 receptors instead of by altering the occupancy of receptors.

关 键 词：甲状腺功能低下 T3受体 肝细胞核 

分 类 号：R581.202[医药卫生—内分泌]