双抗体夹心酶联免疫吸附法测定人干扰素α-2b在豚鼠体液中的含量  

Determination of human interferon α-2b in guinea pig body fluids by double antibody sandwich enzyme-linked immunosorbent assay

作　　者：丁静[1] 陆春燕[2] 陈钢[1] 牧磊[1] 臧林泉[1] 王来友[1] 

机构地区：[1]广东药学院,广州510006 [2]安徽安科生物工程(集团)股份有限公司,合肥230088

出　　处：《中国新药杂志》2010年第16期1455-1458,共4页Chinese Journal of New Drugs

基　　金：广东省自然科学基金(07301575);广东省医学科研基金(B2007090;B2008080);广东药学院师资队伍建设经费

摘　　要：目的：建立双抗体夹心酶联免疫吸附法（ELISA）快速测定豚鼠体液中人干扰素α-2b的浓度,为其新制剂的体内研究奠定基础。方法：对双抗体夹心ELISA法用于豚鼠内耳外淋巴液、脑脊液和血清中人干扰素α-2b浓度的测定进行了方法学考察;豚鼠静脉注射人干扰素α-2b后采集各体液,采用该法测定其中药物浓度。结果：该法在31.25-1 000 pg.mL-1内线性关系良好,板内精密度小于7.8%,板间精密度小于11.9%,各体液中的药物回收率为91%-111%。静脉给药后,药物在内耳外淋巴液和脑脊液中的浓度远远低于血清。结论：该法简单、快捷、灵敏,适用于人干扰素α-2b在豚鼠体内的含量测定及药动学研究。Objective：To establish an analytical method for the determination of human interferon α-2b（IFNα-2b） in body fluids of guinea pig.Methods：The double antibody sandwich enzyme-linked immunosorbent assay（ELISA） was validated for the determination of IFNα-2b concentration in perilymph（PL）,cerebrospinal fluid（CSF） and serum.The body fluids of guinea pigs were collected after intravenous injection of IFNα-2b solution.The concentrations of IFNα-2b in PL,CSF and serum were measured by double antibody sandwich-ELISA.Results：The calibration curves were linear over the concentration range of 31.25~1 000 pg·mL^-1.Within the range,the within-plate and between-plate RSDs were less than 7.8% and 11.9%,respectively.The overall recovery rate of the method was 91%~111%.The concentration levels of IFNα-2b in PL and CSF were much lower than those in serum after intravenous injection.Conclusion：This analytical method is shown to be convenient,rapid and sensitive,and is suitable for the pharmacokinetic study of IFNα-2b in guinea pigs.

关 键 词：人干扰素Α-2B 双抗体夹心酶联免疫吸附法 外淋巴液 脑脊液 血清 

分 类 号：R927.2[医药卫生—药学] R978.7