一种研究重症急性胰腺炎肠道屏障损害的猪模型  

Pig model for intestinal barrier function injury and its mechanism during severe acute pancreatitis

作　　者：邹树[1] 邵洪[1] 田伏洲[1] 黄大熔[1] 闫勇[1] 李小军[1] 尹致良[1] 高晓美[1] 

机构地区：[1]成都军区总医院全军普外中心,610083

出　　处：《中华实验外科杂志》1999年第3期210-211,共2页Chinese Journal of Experimental Surgery

摘　　要：目的介绍一种新的研究（SAP）肠道屏障功能损害的动物模型。方法小型猪无菌手术行中心静脉插管、肠系膜下静脉和门静脉插管、胰管插管、空肠造口和膀胱造瘘。气相色谱法测定尿中乳果糖和甘露醇含量比值反映肠道通透性。高分辨胃肠功能测定仪测量肠道动力。用对氨基马尿酸法测定门静脉血流量反映肠道血供。结果与结论通过和其他研究SAP模型相比较，该模型主要具有如下优点；在清醒状态下连续动态同步观测同一动物在SAP后一段较长时间内（1周或2周）肠道通透性、血供及脑动力等的变化情况，更好地反映这几个因素之间的关系。ObJective To introduce a new severe acute pancreatitis (SAP) medel of studying intestinalbarrier function injury. Methods Cannulations of central vein, portal vein, inferior mesenteric vein and pancreatic duct, jejunostony and cystostomy were per formed on experimental pigs. Urinary content of lactuloseand mannitol was determined by gas chromatography. Intestinal motility was measured by highly discriminatedgastro-intestinal function analyzer. Portal blood flow reflecting intestinal blood supply was measured by usingthe para-aminohippuric acid method. Results & Conclusions Compared with other SAP models, the modelis mainly characterized by continuous and simultaneous observation of intestinal permeabiIity, blall flow andmotility of the same SAP animaI for a period of one or two weeks under conscious state. The relation arnongthe factors could be better investigated.[

关 键 词：胰腺炎 细菌移位 动物模型 肠道屏障损害 

分 类 号：R657.51[医药卫生—外科学]