恶性疟原由海南株cDNA克隆FMPf01分析  

ANALYSIS OF cDNA CLONE (FMPf01) FROM cDNA EXPRESSION LIBRARY OF ERYTHROCYTIC STAGE PLASMODIUM FALCIPARUM (FCC/HN)

作　　者：王萍[1] 李明[1] 谢毅[2] 王燕妮[1] 毕惠祥[1] 殷明[2] 叶韫辉 李英杰[1] 

机构地区：[1]第一军医大学热带病研究室,广州510515 [2]上海复旦大学遗传所国家重点实验室基因合成室

出　　处：《中国人兽共患病杂志》1999年第3期40-42,53,共4页Chinese Journal of Zoonoses

基　　金：全军医药卫生基金!96L034

摘　　要：目的对我室构建恶性疟原虫海南株（FCC／HN）红内期cDNA表达文库阳性克隆（FMPf01）进行分析研究，确定其性质。方法通过计算机软件和免疫印迹法分析FMU01的减基构成、同源性及编码多肽的免疫反应性。结果核苷酸序列分析显示其A＋T构成比高于G＋C，为3．14：1，与基因资料库中疟原虫基因的同源性较低，其最高值为63．8％，Westernblot分析证实其编码多肽可被抗疟原虫免疫血清识别，反应性较强。结论FMPf01编码多肽有望成为疟疾免疫学诊断和疫苗的候选抗原。Aim To sequence and analyze a clone(FMPf01)from cDNA expression library of erythrocytic stage Plas-modium falciparum- Methods Computer programs and Western b1ot were used. Result The FMPfO1 contains high ratio ofA and T. A+T/G+C is 3. l4. The best scores of similarity to the gene sequences of Plasmodium in Gen Bank are between55. 6 % ~63' 8%. The expressed peptide of FMPfO1 reacted with serum from the rabbit immunized with P. falciParnm. Cou-clusion The FMPfO1 might be a candidate antigen for the development of malaria vaccine and immunodiagnostic methods.

关 键 词：疟疾 恶性疟 CDNA 疟原虫 克隆 序列分析 

分 类 号：R531.3[医药卫生—内科学]