Production of a newly isolated Paenibacillus polymyxa biocontrol agent using monosodium glutamate wastewater and potato wastewater  

作　　者：Likun Gu Zhihui Bai Bo Jin Jianyun Zhang Wenying Li Guoqiang Zhuang Hongxun Zhang 

机构地区：[1]Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China [3]Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China [4]School of Earth and Environment Sciences, the University of Adelaide, Adelaide, SA 5005, Australia

出　　处：《Journal of Environmental Sciences》2010年第9期1407-1412,共6页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 30600082);the Knowledge Innovation Program of Chinese Academy of Sciences (No.KSCX2-YW-G-054-2);the Key Technologies R&D Program of China (No. 2008BADA7B01)

摘　　要：A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater （MGW） and potato wastewater （PW） as culture medium. The maximum yield of 6.5 × 10^9 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28℃, pH 6.0-7.5 using the mixture of MGW and PW （1：9）.A phyllosphere bacterial strain EBL-06 was isolated from wheat leaves. The morphology, cultural characteristics, phospholipid fatty acids, physiological and antagonistic fungus activities of this strain were investigated. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the relevant bacteria. The results showed that the isolate EBL-06 was a strain of Paenibacillus polymyxa; this strain performed a high level of antagonistic fungus activity toward a broad spectrum of phytopathogens, such as Botrytis cinerea, Cladosporium cucumerinum, Fusarium spp. The isolate EBL-06 can grow well using monosodium glutamate wastewater （MGW） and potato wastewater （PW） as culture medium. The maximum yield of 6.5 × 10^9 CFU/mL of the isolate EBL-06 anti-fungus biocontrol agent was reached in 15 hr cultivation at 28℃, pH 6.0-7.5 using the mixture of MGW and PW （1：9）.

关 键 词：PHYLLOSPHERE Paenibacillus polymyxa WASTEWATER phospholipid fatty acids biocontrol agent 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治] X792.03[农业科学—植物保护]