Construction of WCB-11:A novel phiYFP arsenic-resistant whole-cell biosensor  

作　　者：Qing Hu ,Li Li,Yanjuan Wang,Wenjuan Zhao,Hongyan Qi,Guoqiang Zhuang Department of Environmental Bio-Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China. 

出　　处：《Journal of Environmental Sciences》2010年第9期1469-1474,共6页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 20707035,20777089);the National High Technology Research and Development Program (863) of China (No. 2007AA06A407)

摘　　要：The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome ofEscherichia coli DH5ct, andphiYFP was introduced into E. coli DH5ct as a reporter gene to construct an arsenic-resistant whole-cell biosensor （WCB-11） in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression ofphiYFP. When strain WCB-11 was exposed to As^3＋ and As^5＋, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome ofEscherichia coli DH5ct, andphiYFP was introduced into E. coli DH5ct as a reporter gene to construct an arsenic-resistant whole-cell biosensor （WCB-11） in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression ofphiYFP. When strain WCB-11 was exposed to As^3＋ and As^5＋, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.

关 键 词：ARSENIC reporter gene whole-cell biosensor yellow fluorescent protein （phiYFP） 

分 类 号：Q813[生物学—生物工程]