腺苷酸活化蛋白激酶α2基因多态性与2型糖尿病糖脂代谢的相关性  

The relationship between AMPKα_2 gene polymorphism and glucolipid metabolism in type 2 diabetes

作　　者：杜彦丽[1] 陈莉明[2] 王肃[1] 

机构地区：[1]天津市第五中心医院内分泌科,300450 [2]天津医科大学代谢病医院

出　　处：《中国糖尿病杂志》2010年第9期668-670,共3页Chinese Journal of Diabetes

摘　　要：目的探讨2型糖尿病患者腺苷酸活化蛋白激酶(AMPK)_(α2)基因单核苷酸多态性(SNP)-rs2143754与糖脂代谢的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)和测序的方法,同时检测生化指标。结果糖尿病组(T2DM)与健康对照组(NC)的基因型构成比差异有统计学意义(P<0.05);T/T基因型糖尿病患者有较低的空腹胰岛素和餐后2小时胰岛素,较高的HOMA-IR(P<0.01),较高的TC、TG、LDL-C和较低的HDL-C(P<0.05);rs2143754基因多态性与糖尿病心血管并发症明显相关(P<0.01),T等位基因是心血管疾病的非独立危险因素。结论 rs2143754具有多态性频率分布,T/T基因型胰岛素抵抗加重,是糖尿病心血管病并发症非独立危险因素。Objective To evaluate the relationship between SNP-rs2143754 of AMPKα2 gene and glucolipid metabolism among Tianjin population with T2DM in China. Methods PCR-RFLP and sequencing were performed. Results There was significant difference in genotype constituent ratio between T2DM and NDM（P〈0.01）. T/T genotype had lower fasting insulin and 2-h insulin, higher HOMA-IR （P〈0. 01）, higher TC, TG, LDL-C, and lower HDL-C （P〈0. 05）. There was significant association between the polymorphism of rs2143754 of AMPKα2 and diabetic cardiovascular complications （P〈0. 01）. T allele was an independent risk factor for diabetic cardiovascular complication. Conclusions The polymorphism of rs2143754 exists among Tianjin population. The insulin resistance of T/T genotes aggravates, which is independent risk factor for diabetic cardiovascular complication.

关 键 词：糖尿病 2型 AMPKα2 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]