二甲双胍和游泳运动对肥胖大鼠不同组织内脏脂肪素蛋白表达和血清水平的影响  

Effects of metformin and swimming exercise on serum visfatin level and visfatin protein expressions in different tissues of obese rats

作　　者：高雅[1] 王长江[1] 代芳[1] 罗莉[1] 贾敬华[1] 胡红琳[1] 沈继龙 

机构地区：[1]安徽医科大学第一附属医院内分泌科,合肥230022 [2]人畜共患病研究所

出　　处：《中国糖尿病杂志》2010年第9期696-700,共5页Chinese Journal of Diabetes

基　　金：安徽省教育厅基金项目(KJ2009B047Z)

摘　　要：目的观察二甲双胍、游泳运动及二者联合干预对肥胖大鼠血清、皮下脂肪、肾周脂肪和肌肉组织内脏脂肪素(Visfatin)蛋白表达水平的影响。方法 SD大鼠分为普食组(ND,n=10)和高脂组(HD,n=60),HD组建成肥胖模型(n=40)后随机分为肥胖对照组(OB),二甲双胍组(MET)、游泳组(SWI)、二甲双胍+游泳组(M+S),各10只。测定干预6周前后各组体重(BW)、空腹血糖(FPG)、甘油三酯(TG)、总胆固醇(TC)、空腹胰岛素(FIns)及血清Visfatin。Western Blot法检测大鼠皮下脂肪、肾周脂肪和肌肉组织各组Visfatin蛋白表达水平。结果 (1)干预前,肥胖模型各组BW、FPG、TG、TC、FIns及Visfatin较ND组升高(P<0.01或P<0.05)。干预后,MET组、SWI组、M+S组BW、TG、TC、FPG、FIns及Visfatin较OB组降低(P<0.01或P<0.05)。(2)肾周脂肪、皮下脂肪及肌肉组织的OB组Visfatin蛋白表达量较ND组增加(P<0.01),以肾周脂肪显著(P<0.05),皮下脂肪与肌肉组织表达量无差异。肾周脂肪和皮下脂肪MET组、SWI组、M+S组较OB组降低,以MAS组显著(P<0.05),MET组与SWI组差异无统计学意义。肌肉组织MET组、SWI组、M+S组与OB组Visfatin蛋白表达无差异,干预组间差异无统计学意义。(3)FPG、FIns与Visfatin水平呈线性正相关相关(P<0.01或P<0.05)。结论 Visfatin主要表达于内脏脂肪,参与肥胖大鼠的糖脂代谢,二甲双胍和游泳运动可下调肥胖大鼠血清、皮下脂肪和肾周脂肪Visfatin的表达,对肌肉组织Visfatin表达无影响。Objective To observe the the effects of metformin, swimming exercise and the combination of both interventions on the serum visfatin and visfatin protein expression in subcutaneous adipose tissue（SAT）, perirenal adipose tissue （PAT） and muscle tissue （MT） of high-fat diet-fed obese rats. Methods 70 SD rats were randomly assigned into normal diet group （ND） （n= 10） and high-fat diet group （HD） （n=60）. Obese model （n=40） was established and obese rats were randomly assigned into obese group （OB）, metformin group （MET）, swinmming exercise group （SWI）, and the metformin plus swimming exercise group （M＋ S）（all n= 10）. Body weight （BW）, fasting plasma glucose（FPG）, triglyceride （TG）, total cholesterol （TC）, fasting insulin （Fins） and fasting visfatin were monitored before and after 6 weeks of treatment. Results BW, FPG, TG, TCH, Fins and visfatin levels of each group in obese model were increased significantly （P〈0.01 or P〈0. 05）. BW, FPG, TG, TC, Fins and visfatin levels were decreased in MET, SWI and M＋S groups after treatment （P〈0. 01 or P〈0.05）.Visfatin protein expression levels in SAT, PAT and MT of obese group, especially that of PAT, were increased as eompared to ND group（P〈0.01 or P〈0.05）. In SAT and PAT, the visfatin expression levels in M＋ S, MET and SWI, especially in M＋S group, were decreased after intervention, visfatin expression levels in MT of M＋S, MET, and SWI groups were decreased with no statistical difference. The levels of FPG and Fins were positively related to the lever of visfatin （P〈0. 01 or P〈0. 05）. Conclusions Visfatin, which preferentially expresses in viscera adipose tissue, may play a role in the glucolipid metabolism of obese rats. Metformin and swimming exercise can down-regulate visfatin protein expression in SAT and PAT but has no influence on that of MT.

关 键 词：二甲双胍 运动 内脏脂肪素 肥胖 

分 类 号：R589.2[医药卫生—内分泌]