两株双歧杆菌的耐氧驯化及生长特性研究  

The oxygen-resistant tames and growth characteristics of two kinds of bifidobacteria

作　　者：韩雪[1] 冯镇[2] 易华西[1] 杜明[1] 张兰威[1] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,哈尔滨150090 [2]东北农业大学食品科学与工程学院,哈尔滨150030

出　　处：《食品科技》2010年第9期37-40,共4页Food Science and Technology

基　　金：国家863项目(2007AA10Z354);哈尔滨工业大学优秀青年教师项目(HITQNJS.2008.055);哈尔滨工业大学创新基金项目(HIT.NSRIF.2009113)

摘　　要：采用含不同营养物的全脂奶粉,通过不同高度培养基溶氧量的不同,对双歧杆菌进行逐步有氧驯化,分析驯化后菌株的生长特性。最终确定经驯化后的两株双歧杆菌均可在兼性厌氧条件下生长,INF与PBA最适生长pH为6.85,在有氧条件下培养16 h菌数分别为1.07×109 cfu/mL与9.72×108 cfu/mL。符合活菌制剂的要求,为双歧杆菌生产提供理论基础。Two kinds of bifidobacteria was tamed to be partial anaerobic by using different height culture which has different content of oxygen and analyzed its growth characteristics.Then using these two oxygen resistent bifidobacteria to ferment soybean milk and skim milk.The results showed these two kinds of bifidobacteria can growth when the oxygen exist.The viable count of PBA and INF obtained to 9.72×108 cfu /mL,1.07×109 cfu/mL separately.The optimal growth pH of PBA and INF were 6.85.

关 键 词：双歧杆菌 耐氧驯化 生长特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]