柔红霉素对培养人视网膜色素上皮细胞核仁组成区相关嗜银蛋白影响的定量分析  

A Quantitative Study of the Effect of Daunoblastina on Argyrophilic Nucleolar Organiser Region-associated Proteins in Cultured Human Retinal Pigment Epithelial Cells

作　　者：王雨生[1] 惠延年[1] 

机构地区：[1]第四军医大学西京医院眼科,西安710032

出　　处：《眼科学报》1999年第2期70-73,共4页Eye Science

基　　金：国家自然科学基金资助;基金号39700154

摘　　要：目的:了解视网膜色素上皮(retinal pigment epithelium,RPE)细胞核仁组成区相关嗜银蛋白(ar-gyrophilic nucleolar organiser region-associated proteins,AgNORs)的表达量及柔红霉素对其影响。方法:将柔红霉素加入培养人RPE细胞的培养液中(终浓度为180μg/L)作用12小时,撤药后继续培养24小时。对培养细胞行AgNORs染色,图像分析染色结果。结果:对照组和柔红霉素组AgNORs面积(μm^2/核)分别为41.61±4.52和13.66±0.65(P<0.01);平均面密度(AgNORs面积与胞核面积之比)(%)为20.37±1.97和13.06±2.44(P<0.01)。结论:柔红霉素抑制体外培养人RPE细胞核仁组成区转录活性可能是其细胞毒作用的机理之一。眼科学报1999;15:70—73。Purpose : To investigate the effect of daunoblastina on argyrophilic nucleolar organiser region-associated proteins (AgNORs) in cultured human retinal pigment epithelium (RPE) cells.Methods:The culture medium of secondary cultured human RPE cells were replaced with a daunoblastina-containing one at a dose of 180μg/L,and the cells were incubated for 12 hr at 37℃. After that, the medium was withdrawn, fresh medium was added and incubation was carried out for an additional 24 hr. AgNORs were studied by using a silver staining technique and image analysis system.Results; The area of intranuclear AgNORs (μm2 /nucleus) in the control and the daunoblastina-treated group were 41.61±4. 52 and 13. 66 ± 0. 65 ( P <0.01), and the area density of intranuclear AgNORs(AgNORs/nucleus area ratio) ( % ) in the two groups were 20.37±1.97 and 13.06±2.44( P <0.01) respectively. Conclusion: Inhibiting nucleolar organiser region transcriptional activity may be partly responsible for the cytotoxicity of daunoblastina on the cultured human RPE cells. Eye Science 1999; 15: 70 - 73.

关 键 词：视网膜色素上皮 柔红霉素 核仁相成区 嗜银蛋白 

分 类 号：R979.14[医药卫生—药品]