间充质干细胞成血管因子受体表达特征及其意义  

The characteristical of angiogenic factor receptor expression in mesenchymal stem cell

作　　者：刘春风[1] 陈亮华[2,3] 杨伟[2,3] 唐俊明[2] 杨建业[1] 王家宁[1] 孔霞[1] 郭凌郧[1] 郑飞[1] 黄永章[1] 

机构地区：[1]郧阳医学院附属人民医院临床医学研究所,十堰442000 [2]郧阳医学院生理学教研室 [3]郧阳医学院第一临床学院

出　　处：《陕西医学杂志》2010年第9期1107-1110,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金(No30700306);郧阳医学院大学生科研项目(NoDXSKY026)

摘　　要：目的:探讨间充质干细胞成血管因子受体表达特征及其意义。方法:采用经典的全骨髓贴壁法培养骨髓源间充质干细胞(MSC),通过成骨、成脂肪等多向诱导分化以及流式细胞分析其表面标记(CD34、CD90、CD29)等鉴定MSC特征;以P1-P17MSC为实验材料,利用RT-PCR的方法检测PDGFR、VEGFR和NRP1的表达。利用Transwell体外迁移体系初步探索VEGF在MSC迁移中的作用,同时利用MTT法评价VEGF对MSC增值的作用。结果:培养的MSC呈现出CD90、CD105强阳性,具有成骨、成脂肪等多向分化能力;强表达PDGFR-β和NRP1、不表达VEGFR(Flk1和Flt1)和PDGFR-α,VEGF促进了MSC的增殖和迁移。结论:VEGF可能通过PDGFR-NRP1促进了MSC的增值和迁移。Objective：To explore the characteristical of angiogenic factor receptor expression in mesenchymal stem cell（MSC）.Methods：MSC culture was performed with the classical adhering method;characteristic of MSC was identified through multi-differentiation and surface marker assay（CD90,CD133,CD34,CD29）;the expression of PDGFR,VEGFR and NRP1 in MSC were analyzed by RT-PCR,the proliferation rate of MSC was measured after VEGF treatment by MTT assay,and the VEGF dose-dependent of MSCs migration were observed by using tanswell in vitro migration assay system.Results：Cultured MSCs had the ability of multi-differentiation and highly expressed CD29 and CD90.MSC showed the characteristical of PDGFR,VEGFR and NRP1.VEGF promoted the proliferation and migration of MSC in a dose or time-dependment maner.Conclusion：VEGF can promote the proliferation and migration of MSC through the PDGFR-NRP1 pathway.

关 键 词：干细胞/生理学 中胚层 血管生成因子/代谢 细胞培养 动物 实验 大鼠 

分 类 号：R331.32[医药卫生—人体生理学]