毛竹细胞壁自发荧光的显微荧光分光光度分析  

Microspectrofluorometric Analysis of Autofluorescence in the Cell Walls of Phyllostachys pubescens Culm 

作　　者：贺新强[1,2] 李素文[1,2] 胡玉熹[1,2] 林金星 

机构地区：[1]中国科学院植物研究所 [2]北京师范大学生物系

出　　处：《Acta Botanica Sinica》1999年第7期711-714,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国际热带木材组织和教育部留学回国人员科研启动基金

摘　　要：利用冰冻切片、荧光显微镜、显微荧光分光光度计、组织化学等方法，观察分析了毛竹（ＰｈｙｌｏｓｔａｃｈｙｓｐｕｂｅｓｃｅｎｓＭａｚｅｌ）茎不同组织在０．１ｍｏｌ／Ｌ氨水、１ｍｏｌ／ＬＮａＯＨ及过氧化氢／冰醋酸混合液处理前后细胞壁自发荧光变化。毛竹茎所有组织在紫外光激发下均产生蓝色荧光；氨水处理后，所有组织荧光强度增加，富含阿魏酸的组织，荧光颜色由蓝色转变为绿色，荧光发射光谱峰值由４７０ｎｍ移至５１０ｎｍ；ＮａＯＨ处理使所有组织荧光强度降低；过氧化氢／冰醋酸混合液处理后，木质化组织仍保持较强的蓝色荧光，而未木质化的组织荧光消失。结果表明，原生木质部导管在维管束分化早期就已木质化；阿魏酸广泛分布于竹笋各种幼嫩组织中，随着毛竹生长、细胞壁木质化的发展其含量下降。此外。Autofluorescence in the cell walls of Phyllostachys pubescens Mazel culms was analyzed by ultraviolet fluorescence microscopy, microspectrofluorometer and histochemical staining before and after respective treatment with ammonia, NaOH and H 2O 2/HAC. All tissues of the bamboo culm showed blue autofluorescence under ultraviolet irradiation. With the treatment of ammonia, the tissues increased their fluorescence intensity, among which those rich in ferulic acid changed their color into green, the peak of fluorescence emission spectra shifted from 470 nm to 510 nm. Nevertheless, the fluorescence intensity of all tissues decreased dramatically after the treatment of NaOH. With H 2O 2/HAC treatment, the lignified tissues remained strong blue autofluorescence, while the fluorescence from unlignified tissues disappeared. The results indicated that protoxylem vessels had already showed lignification before phloem and metaxylem formed in the tissues; ferulic acid was widely distributed in the young tissues of bamboo shoot, the content of which decreased with the progress of the lignification. It is further confirmed that H 2O 2/HAC treatment is an effective method to discriminate the phenolic acids bound to hemicelluloses from the phenolics existed in the lignin molecules.

关 键 词：自发荧光 荧光发射光谱 酚酸 木质素 毛竹 

分 类 号：Q949.714.2[生物学—植物学]