乙烯诱导胡萝卜原生质体凋亡  

Ethylene Induction of Apoptosis in Carrot Protoplasts

作　　者：周军[1] 朱海珍[1] 姜晓芳[1] 戴尧仁[1] 

机构地区：[1]清华大学生物科学与技术系

出　　处：《Acta Botanica Sinica》1999年第7期747-750,共4页Acta Botanica Sinica（植物学报：英文版）

摘　　要：乙烯是一种参与多种重要生理学过程的植物激素。用乙烯利在密闭条件下处理胡萝卜（ＤａｕｃｕｓｃａｒｏｔａＬ．）原生质体（在ｐＨ＞４．１时释放乙烯），发现随着乙烯利浓度增加，细胞死亡率逐渐增高。经乙烯利处理的胡萝卜原生质体出现核内染色质固缩，形成凋亡小体等典型的细胞凋亡的形态学特征。用中性法彗星电泳观测到彗星状的核ＤＮＡ片段的迁移。ＤＮＡ电泳分析观察到细胞凋亡时产生的典型的核小体间ＤＮＡ断裂所形成的梯状条带。结果表明。Ethylene is an important plant hormone involving in various physiological processes. Ethrel which releases ethylene in a solution of pH higher than 4.1, was used to treat the carrot protoplasts. It was found that percentage of cell death kept rising with the increase of ethrel concentration. Characteristics of apoptotic cells, such as nuclear condensation and DNA laddering were observed. Neutral comet assay which detects nuclear DNA fragmentation provided evidence that ethylene induced apoptosis in carrot protoplasts.

关 键 词：细胞凋亡 乙烯 胡萝卜 彗星电泳 DNA梯状条带 

分 类 号：Q942.5[生物学—植物学] Q942.6