菠菜叶片胆碱加单氧酶的纯化及抗体制备  

Purification and Antibody Preparation of Choline Monooxygenase in Spinach Leaves 

作　　者：刘家尧[1,2,3,4] 衣艳君[1,2,3,4] 骆爱玲[1,2,3,4] 马德钦[1,2,3,4] 王学臣[1,2,3,4] 梁峥[1,2,3,4] 

机构地区：[1]中国科学院植物研究所 [2]曲阜师范大学生物系 [3]中国科学院微生物研究所 [4]中国农业大学生物学院

出　　处：《Acta Botanica Sinica》1999年第7期785-787,共3页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金;农业部重点项目

摘　　要：许多高等植物、微生物和动物积累有机物质，如甜菜碱、脯氨酸、多羟基醇等，以适应环境的盐／水胁迫，它们被通称为相容渗透保护剂（ｃｏｍｐａｔｉｂｌｅｏｓｍｏｐｒｏｔｅｃｔａｎｔｓ），因为它们与代谢相容，并能降低离子毒害。甜菜碱是一种分布非常广泛和有效的渗调...A protein from spinach (Spinacia deracea Mill.) leaves was isolated and purified by fractional precipitation and hydroxyapatite column chromatography, and in combination with electrophoresis and electroelution. The protein had a light red_brown color and an absorption peak at 455 nm. SDS_PAGE analysis showed that the protein subunit molecular weight was 45 kD. These characterizations indicates that the isolated protein was choline monooxygenase (CMO), and antisera of the CMO protein from rabbit was obtained. The antisera could cross_react with antibodies of choline oxidase that was isolated from bacteria ( Alcaligenes species), but could not be recognized by antibodies of betaine aldehyde dehydrogenase from higher plants.

关 键 词：胆碱加单氧酶 菠菜 抗体制备 

分 类 号：Q946.54[生物学—植物学] Q946－33