MTT比色法测定促肝细胞生长物质对肝细胞生长的刺激活性  

An MTT colorimetric assay for measurement of the activity of hepatic stimulating substances

作　　者：雷殿良[1] 马霄[1] 顾磊[1] 赵建荣[1] 董联珠[1] 俞永平[1] 

机构地区：[1]中国药品生物制品检定所

出　　处：《微生物学免疫学进展》1999年第3期16-20,共5页Progress In Microbiology and Immunology

摘　　要：本实验建立了用简便的ＭＴＴ比色法对促肝细胞生长物质的促肝细胞增殖作用的测定方法，确定了实验的最适条件。与传统的３ＨＴｄＲ掺入法进行比较的结果显示，ＭＴＴ比色法与３ＨＴｄＲ掺入法测定结果基本相符，灵敏度相近，但消除了同位素的污染，是一个测定促肝细胞生长物质刺激肝细胞增殖活性的简便方法。The stimulating activity of hepatic stimulating substances on the proliferation of hepatic cell line Hep G2 was determined by an MTT colorimetric assay.The test conditions for the assay were optimized.The stimulating index(SI) determined by this assay was well correlated with that measured by 3H TdR incorporation assay.The data indicated that the MTT colorimetric assay is sensitive and the use of the radioactive isotope is avoidable also.It is a suitable method for the determination of the stimulating activity of hepatic stimulating substances.

关 键 词：MTT 肝细胞生长因子 促肝细胞增殖 生物活性 

分 类 号：Q593.1[生物学—生物化学] R392－33[医药卫生—免疫学]