应用蚀斑法和细胞病变法检测乙型脑炎减毒活疫苗病毒的比较  

Comparison of plaque forming unit and cytopathic effect assays for titration of virus titer of Japanese encephalitis attenuated live vaccine

作　　者：贾丽丽[1,2] 王叔桥[1,2] 岳广智[1,2] 王志伟[1,2] 郑铮[1,2] 俞永新[1,2] 王寿贵[1,2] 

机构地区：[1]中国药品生物制品检定所 [2]卫生部成都生物制品研究所

出　　处：《微生物学免疫学进展》1999年第3期28-30,共3页Progress In Microbiology and Immunology

摘　　要：应用蚀斑法（ＰＦＵ）和细胞病变法（ＣＰＥ）检测乙型脑炎减毒活疫苗的病毒滴度，比较结果：①两种方法同时对同一批乙脑活疫苗进行１０次测定，ＣＰＥ滴定结果比ＰＦＵ滴定高１０Ｌｏｇ值，标准差Ｓ＝０１９６，变异系数ＣＶ％＝１９２％；②对不同批乙脑活疫苗检测，仍以ＣＰＥ法高于ＰＦＵ，差值Ｘ＝１２０，标准差Ｓ＝０３１，变异系数ＣＶ％＝２５６％；③ＰＦＵ法对同一批疫苗滴定３０次，乙脑活疫苗参考品滴定１８次，变异系数仅为１４８％和１２７％。结果表明，虽然ＣＰＥ法用于滴定乙脑活疫苗的滴度高于ＰＦＵ法，但蚀斑法系统误差小，重复性好，结果客观可靠，可避免ＣＰＥ法中由于细胞退化和工作人员主观判定造成的系统误差。Two methods of cytopathic effect(CPE) and plaque forming unite assays(PFU)were used in titration of Japanese Encephalitis attenuated live vaccine for compare of their sensibility and reproducibility.The results indicated that there were some differences in titer of JE live vaccine tested by the two methods.When one same lot of JE vaccine was measured repeatedly 10 times,the vaccine titer tested by CPE was 1.0 Log higher than that by PFU,standard deviation(SD)=0.196;When 34 lots of JE live vaccine were tested with CPE and PFU assays respectively,the results of CPE were also higher than PFU,differnece X=1.20,SD=0.31 CV%=25.6%;When one same lot of JE live vaccine was titrated 30 times and one JE reference vaccine 18 times by the PFU methods,the CV% were only 1.48% and 1.27% repectivily.The results showed that although CPE method is more sensitive than PFU method used for titration of JE attenuatecl live vaccine virus,the PFU assay posses many advantages than the CPE method such as more consistency,more reproducibility and objective,It will avoid cell degeneration induced in primary cell culture CPE assay and personal subject with caused some mistakes reading and inconsistency of the results.

关 键 词：乙型脑炎 减毒活疫苗 细胞病变法 蚀斑法 

分 类 号：R373.31[医药卫生—病原生物学] R512.320.1[医药卫生—基础医学]