纤维二糖脱氢酶抑制酚型化合物聚合及促进木素降解的研究  

Cellobiose Dehydrogenase: Inhibition of Polymerization of Phenolic Compounds and Enhancing Lignin Degradation by Ligninases

作　　者：方靖[1] 刘稳[1] 高培基[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《生物化学与生物物理学报》1999年第4期415-419,共5页

基　　金：国家自然科学基金

摘　　要：裂褶菌纤维二糖脱氢酶（ＣＤＨ）可以氧化纤维二糖并还原多种物质， 催化的是一双底物双产物反应， 符合乒乓反应机制。在电子供体纤维二糖存在下，ＣＤＨ 可以还原由豆壳过氧化物酶（ＳＨＰ）氧化多种芳香化合物所生成的产物。ＳＨＰ氧化１羟基苯丙三唑（１ｈｙｄｒｏｘｙｂｅｎｚｏｔｒｉａｚｏｌｅ， ＨＢＴ） 生成的产物对ＳＨＰ有失活作用， 而在纤维二糖存在下，ＣＤＨ可以还原该氧化产物从而阻止其对酶的失活作用。ＣＤＨ可以抑制ＳＨＰ 氧化聚合愈创木酚等酚型化合物的反应。发现ＣＤＨ 可以促进木素酶对木素的降解。对ＣＤＨThe kinetic behavior of cellobiose dehydrogenase (CDH) was investigated by steady state initial velocity studies. Variation in the concentration of one substrate led to changes in K m and V max of the other substrate. The results were consistent with a ping pong mechanism. In the presence of cellobiose, CDH could reduce many oxidized products catalyzed by soybean hull peroxidase (SHP). The oxidation product of 1 hydroxybenzotriazole (HBT) catalyzed by SHP inactivated the enzyme itself; however, CDH could prevent SHP from inactivation by reducing the oxidation product of HBT. CDH could also inhibit the polymerization of phenolic compounds catalyzed by SHP. It was found that the addition of CDH could enhance kraft pulp lignin degradation by ligninases.

关 键 词：纤维二糖脱氢酶 酚型化合物 抑制 木素降解 

分 类 号：TQ352.1[化学工程]