12株酵母菌的亲缘关系分析  

Analysis of Phylogenetic Relationships Among Twelve Yeast Species

作　　者：张平武[1] 罗泽伟[1] 陈力[1] 霍克克[1] 李育阳[1] 

机构地区：[1]复旦大学遗传学研究所遗传工程国家重点实验室,上海200433

出　　处：《生物化学与生物物理学报》1999年第4期477-478,共2页

摘　　要：克隆了９ 株假丝酵母和１ 株克鲁维酵母的２５ ＳｒＤＮＡ 片段， 测定其５′端部分核苷酸序列后与报道的Ｃａｎｄｉｄａ ａｌｂｉｃａｎｓ 及Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ ２５ ＳｒＤＮＡ相应区域的核苷酸序列比较， 采用ｎｅｉｇｈｂｏｒｊｏｉｎｉｎｇ 和ｂｏｏｔｓｔｒａｐ 法分析并绘制系统树。结果提示Ｃａｎｄｉｄａ ｋｅｆｙｒ ＣＢＳ８３４ 与Ｋｌｕｙｖｅｒｏｍｙｃｅｓｃｉｃｅｒｉｓｐｏｒｕｓ ＣＢＳ４８５７Partial sequences of 25 S ribosomal DNA for nine Candida yeast and one Kluyveromyces yeast strains were cloned and determined. We compared them with two published 25 S rDNA sequences of Saccharomyces cerevisiae and Candida albicans . An evolutionary tree of the twelve species was inferred from about 370 sites of 5′ end of 25 S ribosomal DNA using the methods of neighbor joining and bootstrap . The molecular data indicate a very close affinity between Candida kefyr CBS834 and Kluyveromyces cicerisporus CBS4857.

关 键 词：假丝酵母 核糖体 RNA DNA序列 系统树 亲缘关系 

分 类 号：Q949.326.1[生物学—植物学]