水稻亚铁胁迫诱导ADH的基因定位及其遗传分析  

Identification of ADH Gene in Rice Under Fe^(2+) Induction

作　　者：张立平[1] 吴平[1] 祝金明[1] 吴运荣 

机构地区：[1]浙江农业大学生物科学系,杭州310029

出　　处：《Acta Genetica Sinica》1999年第4期359-362,共4页

基　　金：浙江省自然科学基金;美国洛克菲勒基金

摘　　要：籼稻品种IR64与粳稻品种Azucena及其DH群体135个系用于进行FC2+胁迫（250mg／L，pH45）及对照实验，对处理及对照条件下的ADH进行基因定位及遗传分析。结果表明，在Fe2+胁迫条件下，ADH酶的活性大大提高，群体在Fe2+胁迫条件下，表现出低值的超亲现象，分布偏向IR64。单标记分析和最大似然区间作图结果表明，Fe2+胁迫条件下，11号染色体上紧密连锁的3个标记位点RG118－Adh－1－RG1094与ADH活性显著关联（P＜0．001），在3个标记位点上，Azucena基因型ADH活性平均值均显著高于IR64基因型。在Adh－1与RG1094间检测到一LOD值为1177的QTL，该位点对Fe2+胁迫诱导ADH活性变异贡献率为38．2％，加性效应值为4．59。研究表明，应用分子标记分析方法确定有关生理生化代谢过程中的关键酶基因位点是可行的。A double haploid (DH) population consisting of 135 lines derived from a japonica variety, Azucena and an indica variety, IR64 were cultivated hydroponically using excess Fe2+ at the concentration of 250mg L-1 pH, 4.5. ADH gene were identified under Fe2+ stress. The results indicated that the ADH activity was highly increased in parents and population, the distribution of average activity of ADH showed skewness to IR64 in DH lines under Fe2+ stress condition. Molecular marker loci associated with variations in ADH and gene loci for ADH were detected using 175 markers mapped on all 12 chromosomes by single marker loci and interval mapping under Fe2+ stress condition. One gene loci were identified to be flanked by RG1 18-Adh-1-RG1094 on chromosome 11. The detected single QTL could explain more than 38.2% of variation in ADH in population, additive gene action was 4.59.And the results showed that it is possible to map the key enzyme in the process of biochemistry using the molecular marker.

关 键 词：水稻 亚铁胁迫 乙醇脱氢酶 基因定位 

分 类 号：S511.01[农业科学—作物学]