用rep-PCR技术研究中国花生根瘤菌的多样性  

GENETIC DIVERSITY AMONG CHINESE PEANUT RHIZOBIA BY rep PCR ANALYSIS *

作　　者：李俊[1] 徐玲玫[1] 樊蕙[1] 李力[1] 葛诚[1] 杨苏声[2] 

机构地区：[1]中国农业科学院土壤肥料研究所农业部植物营养重点实验室 [2]中国农业大学生物学院微生物系

出　　处：《微生物学报》1999年第4期296-304,共9页Acta Microbiologica Sinica

基　　金：欧盟合作项目

摘　　要：采用细菌基因组重复序列ＰＣＲ技术（简称ｒｅｐＰＣＲ）中常用的ＲＥＰＰＣＲ和ＥＲＩＣＰＣＲ，对从中国１１个省、市的２３个点、２４个花生品种采集的根瘤中分离的５９株花生根瘤菌Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｓｐ．（Ａｒａｃｈｉｓ）进行多样性研究，同时对来自国外的６株花生根瘤菌及１４株参比慢生根瘤菌也进行了比较。得到的低相似性结果表明中国花生根瘤菌基因组存在显著的多样性。ＲＥＰＰＣＲ揭示，在相似性５０％上分为１１个群，而ＥＲＩＣＰＣＲ却得到２４个分群。这两种结果对菌株的分群有差异，暗示这两种短重复序列在慢生根瘤菌基因组中的分布的不同。没有发现菌株间基因组的多样性分布与花生品种、地理来源之间的必然联系。将两者电泳图谱结合并分析，得到介于上述两者间的结果。此结果进一步反映了菌株基因组间存在的多样性。同时还表明ｒｅｐＰＣＲ不仅是研究生物多样性的快速简便方法，还可应用于菌株的鉴别和生态学研究。Repetitive sequences(repetitive extragenic palindromic, and enterobacterial repetitive intergenic consensus ) with the polymerase chain reaction(PCR) were used to fingerprint pure DNA extracted from 79 bradyrhizobial strains. These strains included 59 peanut rhizobia [ Bradyrhizobium sp. ( Arachis) ] isolated from root nodules of 24 peanut ( Arachis hypogaea L) cultivars from 23 sites in 11 provinces in China, 6 peanut rhizobial strains from other nations, and 14 reference strains of other bradyrhizobia. All the strains were clustered at the level of 11%, 2%, and 11,24 clusters were obtained at the similarity of 50% with REP PCR and ERIC PCR fingerprints, respectively. The results showed that significant genomic diversity exists within the peanut rhizobia from China. Also they suggested that there are different distributions of REP and ERIC in genomic DNA of the peanut rhizobia. The combined results, REP plus ERIC data, were found between the both above. The genomic diversity seems not to correlate with their host and geographic origins. Our results supported this technique is a useful tool for genotypic characterization and identification of rhizobia as well as ecological studies.

关 键 词：PCR 花生根瘤菌 多样性 

分 类 号：Q939.114[生物学—微生物学]