质粒RSF1010从大肠杆菌到稀有放线菌头状链轮丝菌和星状诺卡氏菌的接合转移  

CONJUGATIONAL ACTINOMYCETES OF PLASMID RSF101 FROM ESCHERICHIA COLI TO THE RARE ACTINOMYCECES OF NOCARDIA ASTERIODES AND STREPTOVERTICILLUM CAESPITOSUS *

作　　者：李维泉[1] 沈美娟 焦瑞身[1] 赵国屏[2] 

机构地区：[1]中国科学院上海植物生理研究所微生物次生代谢分子调控研究开放实验室 [2]中国科学院上海生物工程研究中心

出　　处：《微生物学报》1999年第4期376-380,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金

摘　　要：１９８７年，ＴｒｉｅｎＣｕｏｔ等人［１］证明穿梭质粒可以在革兰氏阴性的大肠杆菌（Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ）和多种革兰氏阳性细菌之间发生接合转移。在这种转移中质粒需具备大肠杆菌的复制起始位点，同时又具备革兰氏阳性细菌的广宿主范围复制起始位点。转...RSF1010 is a naturally occurring Escherichia coli broad host range plasmid about 8 7 kb in size. It can be mobilized at high frequency between different gram negative bacterial species when transfer functions are available in trans. Following the pioneering work of conjugational transfer of RSF1010 from E.coli to Streptomyces lividans and Mycobacterium smegmatis , the transfer of this plasmid by conjugation from E.coli S17.1 tp two gram positive rare actinomycetes, Nocardia asteroides 3927 and Streptoverticillum caespitosus ATCC27422 was first time reported in this study. Southern blot analysis of the total DNA extracted from the actinomycetes' exconjugants proved that RSF1010 had been transferred from E.coli into the two new hosts and maintained staby in the exconjugants. Meanwhile, partial deletions of RSF1010 replicon loosing its antibiotics resistance makers were readily detected in E.coli. The implenmentation of this observation was discussed.

关 键 词：RFF1010 接合转移 质粒 稀有放线菌 诺卡氏菌 

分 类 号：Q782[生物学—分子生物学] Q939.13