^(15)N标记法研究不同精粗比底物对山羊瘤胃内原虫与细菌间蛋白质微循环的影响  

Effects of different forage to concentrate ratio on nitrogen microcirculation between bacteria and protozoa in goats with ^(15)N-tracer technique

作　　者：刘翔[1] 张红伟[1] 董淑红[1] 王洪荣[1] 熊浩[1] 

机构地区：[1]扬州大学动物科学与技术学院

出　　处：《中国饲料》2010年第16期15-20,共6页China Feed

基　　金：国家自然科学基金项目(2008~2010:30771567)

摘　　要：以4头安装永久瘤胃瘘管的徐淮山羊为试验动物,运用15N稳定性同位素示踪技术,研究了1∶9(A)、3∶7(B)、5∶5(C)、7∶3(D)四种精粗比底物下瘤胃原虫与细菌间蛋白质微循环的影响。结果表明:不同精粗比底物显著影响瘤胃内原虫的吞噬量及微生物蛋白(MCP)的微循环,A、B、C、D四组吞噬N量分别为:18.11×105、21.16×105、20.95×105、30.34×105pg/(mL·h)。每天每头山羊由于原虫的吞噬造成的细菌蛋白循环量分别为:1.09、1.27、1.26、1.82g,其中以C组循环率最大(2.79%),D组蛋白质循环量最高,而B组循环率最低(1.94%),且蛋白质周转量仅为D组的69.78%,可有效减少菌体蛋白循环量。Four goats fitted with rumen cannula were selected as experimental animals,15N-tracer technique was carried out to study the effect of different forage to concentrate ratio on nitrogen microcirculation between bacteria and protozoa in goat.Four kinds of ratio were 1∶9（A）,3∶7（B）,5∶5（C）and 7∶3（D）,respectively.The conclusion were presented as below：The grazing N amount of rumen protozoa on bacterial and MCP microcirculation were affected by the concentrate-to-forage ratio,the grazing amounts of A,B,C and D were presented as follows：18.11×10^5,21.16×10^5,20.95×10^5 pg/（mL·h） and 30.34×10^5 pg/（mL·h）.Extrapolating the assimilation quantity of bacterial protein by ciliates per day per capita,there were 1.09,1.27,1.26 g and 1.82 g respectively,with group C recording the highest circulation rate （2.79 %）,and group D recording the highest bacterial protein circulation amount, while group B recording the lowest circulation rate（1.94 %）and the protein turnover equivalence to 69.78 % of group D,which could effectively reduce the amount of bacterial protein circulation.

关 键 词：15N 原虫 吞噬速率 蛋白循环 

分 类 号：S816.32[农业科学—饲料科学]