纳络酮对东方蝾螈胚胎表皮传导的阻断作用  

THE ANTAGONISTIC EFFECT OF NALOXONE ON THE EPIDERMAL CONDUCTIVITY OF AMPHIBIAN EMBRYOS

作　　者：张小云[1] 曾弥白[2] 蒋婉素 

机构地区：[1]深圳大学生物工程系,深圳518060 [2]中科院上海细胞生物学研究所,上海200031

出　　处：《实验生物学报》1999年第2期157-161,共5页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金;批准号:39770383。

摘　　要：在胚胎发育的一定时期,表皮细胞呈现较强的β-内啡肽阳性免疫反应,而这时期正是表皮传导最活跃的时期。为了探索胚胎表皮传导和β-内啡肽-类阿片样多肽之间是否有关系,本实验采用纳络酮处理,发现表皮传导消失,待纳络酮作用消除后,表皮传导现象又再出现,说明纳络酮在胚胎表皮细胞传导中起了阻断的作用。The immunocytological work revealed that in amphibia β-endorphin is distributed in embryonic epidermis during development with prominent expression at late tail bud stage which coincides with the stage of epidermal conductivity of full strength. To see whether any relationship exists between the presence of β-endorphin and the epidermal conductivity of amphibian embryos . naloxone of 1 and 2μmol/L was used. It has been demonstrated that after 45-60 minutes in the naloxone solution the embryonic epidermal conductivity disappeared and 90-120 minutes after the beginning of naloxone treatment the epidermal conductivity was again recovered. It was suggested that the opiate peptides, such as β-en-dorphin, play some role in the epidermal conductivity of amphibian embryos.

关 键 词：纳络酮 蝾螈 胚胎表皮传导 传导阻滞 

分 类 号：Q132.7[生物学—普通生物学]