VK_3诱导悬浮培养的胡萝卜细胞凋亡  

INDUCTION OF PROGRAMMED CELL DEATH BY MENADIONE IN SUSPENSION CULTURE OF CARROT CELLS

作　　者：颜长辉[1] 陈浩明[1] 戴尧仁[1] 

机构地区：[1]清华大学生物科学与技术系,北京100084

出　　处：《实验生物学报》1999年第2期197-205,共9页Acta Biologiae Experimentalis Sinica

摘　　要：VK_3是一种醌类物质,在体内通过氧化还原产生超氧化自由基。我们的实验发现,VK_3对悬浮培养的胡萝卜细胞及原生质体有致死作用。这种作用是浓度依赖性的。100—800μmol/L的VK_3能引起10%—33%的细胞死亡。当VK_3浓度达到1mmol/L时,死亡率达到100%。DNA电泳分析发现,600和800μmol/L的VK_3处理过的细胞和原生质体产生大小为180bp整数倍的梯状条带。而在更高或更低浓度的处理中则没有观察到这种条带。用TUNEL方法检测,在400—800μmol/L浓度的处理中观察到细胞核DNA的断裂。而在更高或更低浓度的处理中则没有观察到这种断裂。由此我们推测,适当浓度的VK_3能诱导胡萝卜细胞及原生质体凋亡。Menadione (VK,), a quinone that undergoes redox cycles leading to the formation of superox-ide radicals, was found to induce cell death in suspension culture of carrot cells. The effect of menadione was in a dose-dependent manner. 100 -800μmol/L menadione caused 10-33 percent cell death. When concentration of menadione reached 1 mmol/L, 100 percent of cell death was observed. DNA cleavage, a hallmark of apopto-sis was further studied. DNA ladders were ob- served in cells treated with 600 and 800μmol/L menadione but not with lower concentration treatments where only very low percentage of cell death was found. There was no DNA ladders in the cells treated with 1 mmol/L menadion indicating that necrosis may occur. In situ detection of nuclear DNA fragmentation by TUNEL reaction revealed fragmented nuclear DNA in cells treated with 100 - 800μmol/L menadion but not in cells treated with 1 mmol/L menadione.

关 键 词：细胞凋亡 胡萝卜 维生素K3 细胞悬浮培养 

分 类 号：Q942.5[生物学—植物学]