壁虎醇提物抗肿瘤及增效减毒作用的研究  

Study on the Antitumor Effects and Synergism and Attenuation Effects of Gecko Alcohol Extract

作　　者：王晓兰[1] 皓恒友 王建刚[1] 李瑞芳[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003 [2]洛阳市卫生学校生理教研室,河南洛阳471000

出　　处：《中药材》2010年第8期1213-1216,共4页Journal of Chinese Medicinal Materials

基　　金：河南省医学科技攻关计划项目(200903109)

摘　　要：目的:研究壁虎醇提物对S180小鼠的抗肿瘤作用及其对化疗药物环磷酰胺(CTX)的增效减毒作用。方法:以S180荷瘤小鼠为模型,观察不同剂量的壁虎醇提物的抑瘤率,ELISA法测定小鼠血清TNF-α的含量。腹腔注射壁虎醇提物与CTX(20 mg/kg)联合用药12 d后,检测各组小鼠的抑瘤率、外周血白细胞数、脾指数、胸腺指数等指标,分析壁虎醇提物对CTX的增效减毒作用。结果:不同剂量的壁虎醇提物(0.6、1.2、2.4 g/kg)均可抑制S180肉瘤在昆明小鼠体内生长,抑瘤率分别为44.88%、63.94%、69.53%,对小鼠血清TNF-α含量影响不明显。单用CTX组抑瘤率为46.17%,联用壁虎醇提物低、中、高剂量组的抑瘤率分别为56.93%、67.15%、70.24%,与单用CTX组抑瘤率比较(P<0.01)。壁虎醇提物各组联用CTX,与单用CTX组比较,可不同程度地升高荷瘤小鼠的白细胞数(P<0.01)和胸腺指数、脾指数(P<0.05)。结论:壁虎醇提物对S180荷瘤小鼠具有抗肿瘤作用,对化疗药物CTX具有增效减毒作用。Objective： To investigate the antitumor effects of Gecko alcohol extract and its synergism and attenuation effects on CTX.Method： S180-bearing mice were given Gecko alcohol extract via intravenous injection,the tumor inhibitory rate and the levels of serum TNF-α of mice were detected.After inoculation of S180 tumor,the synergism and attenuation effects of Gecko alcohol extract on CTX were observed.After 12 days treatment,the tumor inhibitory rate,the count of peripheral white blood cells,index of thymus and spleen were calculated.Results： Gecko alcohol extract in different dose（0.6,1.2,2.4 g/kg）inhibited the growth of S180 sarcoma in KM mice.The tumor inhibitory rates of 0.6,1.2,2.4 g/kg Gecko alcohol extract were 44.88%,63.94%,69.53%,respectively.However,the levels of serum TNF-α of mice not changed.The tumor inhibitory rates of intravenous administration of 0.6,1.2,2.4 g/kg Gecko alcohol extract combined with CTX（20 mg/kg） were 56.93%,67.15%,70.24%,which were higher than that of CTX administration alone（41.71%）.Compared with those in CTX group,the count of WBC（P〈0.01）,the indexes of thymus and spleen（P〈0.05） were significantly elevated in all Gecko alcohol extract and CTX combination groups.Conclusion： Gecko alcohol extract has anti-tumor effects in vivo and attenuation effects on CTX.

关 键 词：壁虎醇提物 S180小鼠 肿瘤化疗 增效减毒 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]