关木通不同炮制品中马兜铃酸A的含量测定及其对大鼠肾功能的急性损伤试验  

The Determination of Aristolochic Acid A in Different Processed Aristolochia manshuriensis and the Test of Influence about Renal Function in Rats

作　　者：潘金火[1] 严国俊[1] 宋娟[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《中药材》2010年第8期1228-1233,共6页Journal of Chinese Medicinal Materials

摘　　要：目的:通过研究,探讨能显著降低关木通中马兜铃酸含量,减轻其肾毒性的炮制方法与炮制机理。方法:采用传统的炮制工艺对关木通药材进行炮制,制得29种炮制品,连同生品共30种样品,通过高效液相色谱法测定了各样品中马兜铃酸A的含量;通过大鼠急性肾损伤试验,考察了代表性样品对动物肾功能的影响。结果:石灰水煮、石灰水蒸、甘草汁煮、黑豆汁煮、小苏打水煮及滑石粉炒6种样品中马兜铃酸A的含量均有显著降低(降低幅度30%以上),其他炮制品中马兜铃酸A的含量也有一定程度的降低,但降低幅度差异较大。毒理试验结果表明,与生品相比,上述6种炮制品均能显著减轻对动物肾功能的损害,减轻程度与其中的马兜铃酸A含量似有一定的相关性。结论:以关木通为代表的含马兜铃酸中药可以通过适宜的炮制方法降低其马兜铃酸的含量,减轻其对动物肾功能的损害。Objective： To study and approach the processing methods and mechanism which can markedly reduce the content of aristolochic acid in Aristolochia manshuriensis and lighten the nephrotoxicity of aristolochic acid.Methods： A traditional ＂attenuation＂ processing method was used and 30 types of samples which contain one crude and 29 types of processed sample were obtained.The contents of aristolochic acid A in every sample were determined by HPLC.According to the Rat＇s acute renal injury test,the influence of animal＇s renal function was investigated for representative samples.Results： The content of aristolochic acid in six types of samples depressed markedly（30% or more depressed） which processing with boiling in the limewater,steaming with limewater,boiling in the juice of liquorice,boiling in the decoction of black soybean,boiling in the soda water and stir-baked with talcum powder,the content of aristolochic acid in other processed samples also depressed with a large discrepancy.The toxicology test results showed that the above-mentioned 6 samples all can relieve renal injury of rats.There could be some associativity between the degree of renal injury relieving and the content of aristolochic acid A in the samples.Conclusion： The content of aristolochic acid can be reduced and the nephrotoxicity for animals can be lightened with some eligible processing methods for the traditional Chinese medicines containing aristolochic acid with the representative of Aristolochia manshuriensis.

关 键 词：关木通 炮制品 含量测定 毒理研究 

分 类 号：R283[医药卫生—中药学] R285[医药卫生—中医学]