应用微卫星DNA标记对BWEL-SPF鸡种群的群体遗传学分析  

Population Genetic Variation in BWEL-SPF Chickens Inferred from Microsatellite DNA Markers

作　　者：肖兵兵[1] 韩凌霞[1] 牛成明 司昌德[1] 韩建林[2,3] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所实验动物中心兽医生物技术国家重点实验室,哈尔滨150001 [2]中国农业科学院-国际家畜研究所畜禽牧草遗传资源联合实验室 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2010年第9期106-111,共6页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省科技攻关项目(PC06S17);黑龙江省自然科学基金(C2007-06);兽医生物技术国家重点实验室基本科研业务费项目合同(NKLVBP2008)

摘　　要：本研究利用14个鸡微卫星DNA标记位点,采用DNA测序仪和荧光素标记分子内标,建立了鸡分子遗传学的检测方法,对F15世代BWEL-SPF鸡种群8个家系群体内和群体间的遗传结构进行了分析。结果表明,被检位点35.7%(5/14)的基因型趋于纯合,家系内等位基因的变异程度显著降低。F-统计量检验结果表明,家系间的遗传分化均达到了极显著水平(P<0.001),群体间的变异占到了总遗传变异的12.9%。Using 14 microsatellite loci,we analyzed the genetic variation of eight BWEL-SPF chicken families in F15 generation.The results showed that,due to the inbreeding over 14 generations following mating within families,a number of locus（35.7%,5/14） had been fixed with single alleles or approaching to fixation with single predominant alleles.The genetic variation in general had been reduced significantly within families.However,the change of mating scheme from within families to between families since the F15 generation could have converted the positive inbreeding FIS values to negative out-breeding FIS estimates in most families（7/8） with four of them significantly deviated from zero（P〈0.05）,indicating a considerable compromise in inbreeding within the families.Significant tests for the pair-wise FST estimates indicated highly significant genetic differentiations between the eight families（P〈0.001）.There was 12.9% of the total genetic variation present among the families,implying the presence of significant genetic differentiation once present among the families over 14 generations followed within-family selection and breeding.

关 键 词：微卫星DNA标记 SPF鸡 遗传变异 

分 类 号：S813.3[农业科学—畜牧学]