丝素蛋白与胎盘间充质干细胞生物相容性的实验研究  

Study on biocompatibility of silk fibroin with placenta mesenchymal stem cells

作　　者：苗宗宁[1,2] 李芳[2] 吕国忠 张学光[2] 

机构地区：[1]无锡第三人民医院细胞实验室,214041 [2]苏州大学江苏省干细胞研究重点实验室,215007

出　　处：《国际生物医学工程杂志》2010年第4期220-223,I0003,共5页International Journal of Biomedical Engineering

摘　　要：目的 探讨丝素蛋白（SF）材料与胎盘间充质干细胞（PMSCs）的生物相容性.方法 运用SF溶液包被的培养瓶培养PMSCs,流式细胞术分析其表型并对其定向分化潜能进行探讨;PMSCs置于SF膜材料培养后通过扫描电镜观察细胞形态变化.结果 用SF溶液包被的培养瓶培养的PMSCs,其生长特性、表面标志、多向分化潜能无明显变化;PMSCs在SF膜材料上生长良好,培养8 d时材料上细胞伸展增殖,分泌大量颗粒状、网状基质物质,材料间隙被基质填满.结论 SF材料不影响PMSCs的生长特性、表面标志和多向分化潜能,具有良好的生物相容性.Objective To study the biocompatibility of silk fibroin （SF） with placenta mesenchymal stem cells （PMSCs）.Methods PMSCs were cultured in the SF coating flasksand and its phenotype was analyzed by flow cytometry,while its adipogenic,chondrogenic,and osteogenic differentiation potential were determined by staining.The growth condition was then observered under the electric microscope.Results After being cultured in the SF film,the growth characteristics,surface markers,multi-differentiation capacity of PMSCs did not change.The electric microscope observation results showed that PMSCs well proliferated in the SF film after been co-cultured for 8 days,and large amounts of granular was secreted,and mesh matrix substances well filled in the material clearance.Conclusion SF has good biocompatibility by showing no effects on the PMSCs growth characteristics,surface markers and multi-differentiation capacity.

关 键 词：丝素蛋白 胎盘间充质干细胞 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] Q813[医药卫生—基础医学]