化瘀理肺方对肺纤维化大鼠治疗作用的研究  

The Research About Therapy of Huayulifei on Rats with Bleomycin-induced Pulmonary Fibrosis

作　　者：王英田[1] 王琴[2] 刘世青[1] 李长生[1] 

机构地区：[1]山东大学附属千佛山医院,山东济南250014 [2]山东大学医学院,山东济南250012

出　　处：《中华中医药学刊》2010年第9期1908-1911,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：山东省科技攻关课题(2006GG2302030)

摘　　要：目的:探讨化瘀理肺方对博莱霉素致大鼠肺间质纤维化的治疗作用及机制。方法:将大鼠随机分为对照组、模型组、中药组、激素组,采用博莱霉素经气管内插管注入方法(5mg/kg)建立肺间质纤维化模型,对照组注入生理盐水(0.25mL/只)作为正常对照,从造模后的第2天起中药组给予化瘀理肺方药物灌胃(13410mg/kg);对照组、模型组予生理盐水灌胃(2mL/只);激素组予氢化可的松琥珀酸钠腹腔注射(25mg/kg),于给药后的第14天、28天分别处死,取肺组织行苏木素-伊红(HE)染色观察病理变化情况,免疫组化法测定肺组织中Smad3、Smad7蛋白的表达情况以及应用凝胶酶谱法检测MMP-2/9的活性。结果:中药组可见炎性细胞较少,肺泡结构基本正常,肺泡炎与肺纤维化较轻。中药组Smad3蛋白表达明显低于模型组和激素组;Smad7蛋白表达明显高于模型组和激素组;中药组MMP-2/9的活性明显低于模型组和激素组。结论:化瘀理肺方可明显减轻大鼠肺泡炎和肺纤维化的程度,可能通过Smad信号通路从而影响MMP-2/9的活性发挥作用。Objective：To evaluate the therapeutic effect s and mechanisms of Huayulifei on blemycin-induced pulmonary interstitial fibrosis in rats.Methods：The rats were randomly divided into control group,model group,Huayulifei group and hydrocortisone group. Rat pulmonary fibrosis was induced by blemycin（5mg/kg） in experimental groups,while 0.9% saline in control group.Treatments were started from the next day with a daily oral Huayulifei（13410mg/kg）in Huayulifei group,intraperitoneal Hydrocortisone sodium succinate（25mg/kg）in Hydrocortisone group,and oral saline in control group and model group,respectively.Rats were killed at14,and 28 days after bleomycin, lungs isolated, Hematoxylin and eosin-staining,and expression of Smad3、Smad7 protein was measured by immunohistochemical technique, the activities of MMP2/9 was measured by gelatin zymography.Results： In the Huayulifei group having little inflammatory cells,and the alveoli structure was nearly normal,and the most of the airsacculitis and fibrosis of lung was mild. In Huayulifei group the expression of Smad3 protein was significantly lighter than that in model group and hydrocortisone group,while the expression of Smad7 protein was higher;the activities of MMP2/9 was significantly ligher than that in model group and hydrocortisone group.Conclusion： Huayulifei can ameliorate blemycin-induced pulmonary fibrosis in rats,and It may partly work by affecting the expression of Smad signal pathway in lung tissue to decrease the activation of MMP2/9.

关 键 词：肺间质纤维化 化瘀理肺方 Smad3/7 MMP2/9 

分 类 号：R285.5[医药卫生—中药学]